NDLI: Isolation and molecular characterization of multidrug-resistant halophilic bacteria from shrimp farm effluents of Parangipettai coastal waters

Content Provider	Springer Nature Link
Author	Sundaramanickam, Arumugam Suresh Kumar, Poominathan Kumaresan, Saravanan Balasubramanian, Thangavel
Copyright Year	2015
Abstract	Multidrug resistance of heterotrophic bacteria isolated from an aquaculture farm effluent in Parangipettai, at the southeastern coast of India, was investigated. In the initial screening, 27 antibiotic-resistant strains were isolated. All the strains were tested for antibiotic susceptibility against chloramphenicol with varying concentrations. From these, two highly resistant strains, i.e. S1 and S5, were isolated. The selected strains were identified by 16S ribosomal RNA (rRNA) sequencing techniques and confirmed as Bacillus pumilus and Bacillus flexus. Both the antibiotic-resistant strains were further utilized for multidrug susceptibility test by using various antibiotics. These two strains showed antibiotic resistance to 14 of 17 antibiotics tested. Both microdilution assay and well assay methods were used to determine the minimal inhibitory concentration (MIC) for the sensitive strains. Both the tests were shown to be almost similar. Our study highlights the occurrence of multidrug-resistant bacteria in the shrimp farm effluents.
Starting Page	11700
Ending Page	11707
Page Count	8
File Format	PDF
ISSN	09441344
Journal	Environmental Science and Pollution Research
Volume Number	22
Issue Number	15
e-ISSN	16147499
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-04-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antibiotic resistance Shrimp farm effluents Minimal inhibitory concentration (MIC) Bacillus pumilus Bacillus flexus Environment Environmental Chemistry Ecotoxicology Environmental Health Atmospheric Protection/Air Quality Control/Air Pollution Waste Water Technology Water Pollution Control Water Management Aquatic Pollution
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine

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