NDLI: Cross-platform comparative analyses of genetic variation in amino acid content in potato tubers

Content Provider	Springer Nature Link
Author	Carre Quintero, Natalia Undas, Anna Bachem, Christian W. B. Mumm, Roland Visser, Richard R. G. F. Bouwmeester, Harro H. J. Keurentjes, Joost J. J. B.
Copyright Year	2014
Abstract	Many of the biochemical pathways for plant amino acid metabolism are known and, at least in model species, most of the genes encoding the biosynthetic enzymes have been identified. How the accumulation of amino acids is regulated is much less well understood and for this genetic analysis can be instrumental. In potato, the nutritional value of the tubers is often determined by the content of essential amino acids such as lysine, tyrosine, methionine and cysteine. Better insight into the genetic determinants underlying the variation in amino acid accumulation in potato could support efforts to improve tuber nutritional quality by breeding. In this study, we used a diploid potato mapping population to explore the genetic basis of amino acid content. Hereto, we compared the use of one non-targeted and two targeted analytical approaches for amino acid analysis, allowing the evaluation of the robustness of amino acid quantification and the number and strength of detected quantitative trait locis (QTLs) across the different analytical platforms. Assessment of the three methodologies revealed a comparable detection of amino acids using non-targeted and targeted approaches. QTL detection across the different analytical platforms was similar, although slight differences in strength and explained variance were observed. The QTL regions were subsequently studied to provide candidate genes for the genetic regulation of amino acid accumulation in potato. Our results are discussed in the context of the detection of amino acid variation and its implications for the identification of QTLs.
Starting Page	1239
Ending Page	1257
Page Count	19
File Format	PDF
ISSN	15733882
Journal	Metabolomics
Volume Number	10
Issue Number	6
e-ISSN	15733890
Language	English
Publisher	Springer US
Publisher Date	2014-04-26
Publisher Institution	Metabolomics Society
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amino acids Genetic variation Amino acid analysis Quantitative trait loci (QTL) Metabolomics Potato Biochemistry Molecular Medicine Cell Biology Developmental Biology Biomedicine
Content Type	Text
Resource Type	Article
Subject	Endocrinology, Diabetes and Metabolism Clinical Biochemistry Biochemistry

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