NDLI: Purinergic receptors expressed in human skeletal muscle fibres

Content Provider	Springer Nature Link
Author	Bornø, A. Ploug, T. Bune, L. T. Rosenmeier, J. B. Thaning, P.
Copyright Year	2011
Abstract	Purinergic receptors are present in most tissues and thought to be involved in various signalling pathways, including neural signalling, cell metabolism and local regulation of the microcirculation in skeletal muscles. The present study aims to determine the distribution and intracellular content of purinergic receptors in skeletal muscle fibres in patients with type 2 diabetes and age-matched controls. Muscle biopsies from vastus lateralis were obtained from six type 2 diabetic patients and seven age-matched controls. Purinergic receptors were analysed using light and confocal microscopy in immunolabelled transverse sections of muscle biopsies. The receptors P2Y$_{4}$, P2Y$_{11}$ and likely P2X$_{1}$ were present intracellularly or in the plasma membrane of muscle fibres and were thus selected for further detailed morphological analysis. P2X$_{1}$ receptors were expressed in intracellular vesicles and sarcolemma. P2Y$_{4}$ receptors were present in sarcolemma. P2Y$_{11}$ receptors were abundantly and diffusely expressed intracellularly and were more explicitly expressed in type I than in type II fibres, whereas P2X$_{1}$ and P2Y$_{4}$ showed no fibre-type specificity. Both diabetic patients and healthy controls showed similar distribution of receptors. The current study demonstrates that purinergic receptors are located intracellularly in human skeletal muscle fibres. The similar cellular localization of receptors in healthy and diabetic subjects suggests that diabetes is not associated with an altered distribution of purinergic receptors in skeletal muscle fibres. We speculate that the intracellular localization of purinergic receptors may reflect a role in regulation of muscle metabolism; further studies are nevertheless needed to determine the function of the purinergic system in skeletal muscle cells.
Starting Page	255
Ending Page	264
Page Count	10
File Format	PDF
ISSN	15739538
Journal	Purinergic Signalling
Volume Number	8
Issue Number	2
e-ISSN	15739546
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-11-04
Publisher Institution	International Purine Club
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	P2Y receptors P2X receptors Type 2 diabetes Muscle metabolism Neurosciences Human Physiology Biomedicine general Cancer Research Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Cellular and Molecular Neuroscience

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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