NDLI: A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa

Content Provider	Springer Nature Link
Author	Lin, Chien Yuan Li, Quanzi Tunlaya Anukit, Sermsawat Shi, Rui Sun, Ying Hsuan Wang, Jack P. Liu, Jie Loziuk, Philip Edmunds, Charles W. Miller, Zachary D. Peszlen, Ilona Muddiman, David C. Sederoff, Ronald R. Chiang, Vincent L.
Copyright Year	2016
Abstract	Class III peroxidases are members of a large plant-specific sequence-heterogeneous protein family. Several sequence-conserved homologs have been associated with lignin polymerization in Arabidopsis thaliana, Oryza sativa, Nicotiana tabacum, Zinnia elegans, Picea abies, and Pinus sylvestris. In Populus trichocarpa, a model species for studies of wood formation, the peroxidases involved in lignin biosynthesis have not yet been identified. To do this, we retrieved sequences of all PtrPOs from Peroxibase and conducted RNA-seq to identify candidates. Transcripts from 42 PtrPOs were detected in stem differentiating xylem (SDX) and four of them are the most xylem-abundant (PtrPO12, PtrPO21, PtrPO42, and PtrPO64). PtrPO21 shows xylem-specific expression similar to that of genes encoding the monolignol biosynthetic enzymes. Using protein cleavage-isotope dilution mass spectrometry, PtrPO21 is detected only in the cell wall fraction and not in the soluble fraction. Downregulated transgenics of PtrPO21 have a lignin reduction of ~20 % with subunit composition (S/G ratio) similar to wild type. The transgenics show a growth reduction and reddish color of stem wood. The modulus of elasticity (MOE) of the stems of the downregulated PtrPO21-line 8 can be reduced to ~60 % of wild type. Differentially expressed gene (DEG) analysis of PtrPO21 downregulated transgenics identified a significant overexpression of PtPrx35, suggesting a compensatory effect within the peroxidase family. No significant changes in the expression of the 49 P. trichocarpa laccases (PtrLACs) were observed.
Starting Page	1
Ending Page	18
Page Count	18
File Format	PDF
ISSN	16142942
Journal	Tree Genetics & Genomes
Volume Number	12
Issue Number	2
e-ISSN	16142950
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-03-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lignin polymerization Populus trichocarpa Lignin peroxidase LC-MS/MS Lignin systems biology Forestry Plant Genetics & Genomics Plant Breeding/Biotechnology Tree Biology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Horticulture Forestry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Analysis of the genetic variation in growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa provenances

A computational study of the dynamics of LTR retrotransposons in the Populus trichocarpa genome

Genetic linkage mapping in aspen (Populus tremula L. and Populus tremuloides Michx.)

Kunitz-type trypsin inhibitor gene family in Arabidopsis and Populus trichocarpa and its expression response to wounding and herbivore in Populus nigra

A survey of Gypsy and Copia LTR-retrotransposon superfamilies and lineages and their distinct dynamics in the Populus trichocarpa (L.) genome

SNP discovery, gene diversity, and linkage disequilibrium in wild populations of Populus tremuloides

Transcriptome characterization and detection of gene expression differences in aspen (Populus tremuloides)

Molecular evolution of glutathione peroxidase (Gpx) family genes in desert poplar (Populus euphratica Oliv.)

Genome-wide analysis of the LEA (late embryogenesis abundant) protein gene family in Populus trichocarpa

A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa

Similar Documents

Analysis of the genetic variation in growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa provenances

A computational study of the dynamics of LTR retrotransposons in the Populus trichocarpa genome

Genetic linkage mapping in aspen (Populus tremula L. and Populus tremuloides Michx.)

Kunitz-type trypsin inhibitor gene family in Arabidopsis and Populus trichocarpa and its expression response to wounding and herbivore in Populus nigra

A survey of Gypsy and Copia LTR-retrotransposon superfamilies and lineages and their distinct dynamics in the Populus trichocarpa (L.) genome

SNP discovery, gene diversity, and linkage disequilibrium in wild populations of Populus tremuloides

Transcriptome characterization and detection of gene expression differences in aspen (Populus tremuloides)

Molecular evolution of glutathione peroxidase (Gpx) family genes in desert poplar (Populus euphratica Oliv.)

Genome-wide analysis of the LEA (late embryogenesis abundant) protein gene family in Populus trichocarpa

A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa