NDLI: Whole transcriptome analysis of three leaf stages in two cultivars and one of their F$_{1}$ hybrid of Camellia sinensis L. with differing EGCG content

Content Provider	Springer Nature Link
Author	Lin, Jinke Wilson, Iain W. Ge, Guoping Sun, Guiling Xie, Fuliang Yang, Yanfang Wu, Liangyu Zhang, Baohong Wu, Jianqiang Zhang, Yue Qiu, Deyou
Copyright Year	2017
Abstract	Tea (Camellia sinensis (L.) O. Kuntze) is one of the most beneficial beverages due to its health attributes. Whole leaf transcriptome analysis of three leaf stages of C. sinensis has the potential to reveal candidate genes associated with these major beneficial compounds. Epigallocatechin-3-gallate (EGCG) is one of the most important bioactive catechin (antioxidant) in tea drink derived. EGCG has many important physiological functions and positive effects on human health, such as anticancer, antioxidation, controlling blood pressure, and blood sugar content. Although the major metabolic pathways of tea-specific compounds have been established, knowledge of the genes involved in the regulation and synthesis of EGCG in C. sinensis is limited. To gain insight into the molecular mechanisms that regulate EGCG levels, leaf transcriptomes were sequenced at three developmental stages (bud, second leaf, and fourth leaf) of two C. sinensis cultivars and one F$_{1}$ hybrid line #1005 that possess varying EGCG levels. Approximately 470.9 M of quality controlled-filtered sequencing paired-end reads corresponding to 47.09 G nucleotides were obtained from which 130,762 unique sequences were identified. Genes whose expression correlated with changes in EGCG levels were identified, which may be associated with EGCG content. Prediction of the potential targets of microRNAs (miRNAs), the specific subset of long noncoding RNAs (LncRNAs), and mRNA-like noncoding RNAs (mlncRNAs) indicated that miRNAs may play a role in the expression regulation of the genes involved in EGCG levels.
Starting Page	1
Ending Page	14
Page Count	14
File Format	PDF
ISSN	16142942
Journal	Tree Genetics & Genomes
Volume Number	13
Issue Number	1
e-ISSN	16142950
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-01-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Camellia sinensis Tea plant Transcriptome Leaf Epigallocatechin-3-gallate Gene Expression regulation Forestry Plant Genetics & Genomics Plant Breeding/Biotechnology Tree Biology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Horticulture Forestry

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