NDLI: Identification and expression profiling of drought-regulated genes in mulberry (Morus sp.) by suppression subtractive hybridization of susceptible and tolerant cultivars

Content Provider	Springer Nature Link
Author	Gulyani, Vibha Khurana, Paramjit
Copyright Year	2011
Abstract	Mulberry, the backbone of sericulture industry, is a rainfed crop, and its biomass production is affected adversely under drought conditions. In this study, genes expressed differentially during drought stress response have been examined by PCR-Select subtractive hybridization. The sensitive and tolerant genotypes were identified based on physiological evaluation by determination of proline content, electrolyte leakage, and measurement of relative water content. In total, 1,920 clones were sequenced, representing 208 contigs and 151 singletons. The expressed sequence tags generated from this subtracted cDNA library comprises a broad repertoire of stress-responsive genes, which contribute to the process of drought tolerance in mulberry. Additionally, 23% of the cDNA library is represented by transcripts of unknown function. The expression of a select number of these drought-inducible genes was studied based on cDNA macroarray and Northern blot analyses. In order to unravel the crosstalk with other abiotic stresses, expression profile of Arabidopsis homologs of selected genes in response to a wide range of different stresses was studied using Genevestigator as a reference expression database. The results of this study show that subtractive hybridization coupled with validation steps for differential screening is an effective method for identification of stress/drought-induced genes in plants with limited sequence information available.
Starting Page	725
Ending Page	738
Page Count	14
File Format	PDF
ISSN	16142942
Journal	Tree Genetics & Genomes
Volume Number	7
Issue Number	4
e-ISSN	16142950
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-02-20
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mulberry Subtractive hybridization Transcriptome analysis Water stress Plant Breeding/Biotechnology Biotechnology Tree Biology Forestry Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Horticulture Forestry

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