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| Content Provider | Springer Nature Link |
|---|---|
| Author | Li, Yidan Shan, Xiaohui Liu, Xiaoming Hu, Lanjuan Guo, Wanli Liu, Bao |
| Copyright Year | 2008 |
| Abstract | We report here that by using a modified scoring criterion, the methylation-sensitive amplified polymorphism or MSAP marker can be used effectively to detect polymorphism in DNA methylation patterns within and among populations of a perennial wild barley species, Hordeum brevisubulatum. Twenty-four selected individual genotypes representing four natural populations of H. brevisubulatum distributed in the Songnen Prairie in northeastern China were studied. The utility of MSAP was evidenced by its detection of high levels of polymorphism in DNA methylation patterns between individuals within a given population, and the clear inter-population differentiation in methylation patterns (methylation-based epigenetic population structure) revealed among the four populations. The resolving power of MSAP to detect DNA methylation polymorphism was found to be comparable with that of a retrotransposon-based sequence-specific amplified polymorphism marker, or SSAP, to detect genetic polymorphism in the same set of plants, suggesting that MSAP with a modified scoring criterion can be used efficiently to detect DNA methylation polymorphism and assess epigenetic population structure in natural plant populations. |
| Starting Page | 927 |
| Ending Page | 930 |
| Page Count | 4 |
| File Format | |
| ISSN | 09123814 |
| Journal | Ecological Research |
| Volume Number | 23 |
| Issue Number | 5 |
| e-ISSN | 14401703 |
| Language | English |
| Publisher | Springer Japan |
| Publisher Date | 2008-01-16 |
| Publisher Place | Japan |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | DNA methylation polymorphism Epigenetic population structure MSAP Hordeum brevisubulatum Forestry Behavioural Sciences Evolutionary Biology Zoology Plant Sciences Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology, Evolution, Behavior and Systematics |
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