NDLI: A strategy for screening microbial strains with lipolytic specificity toward monoacylglycerols

Content Provider	Springer Nature Link
Author	Huang, Ji Yuan Wang, Teng Hsu Su, Nan Wei Lee, Min Hsiung
Copyright Year	2009
Abstract	For easy obtaining the microorganisms with lipolytic specificity toward monoacylglycerols, we developed a simple and effective method to isolate the objective strains. This method employed a nile-blue agar-plate culture containing mono- and tri- acylglycerols for microorganism screening and selected the desired microorganisms by analysis of free fatty acid contents in lipid extracts obtained from culture broth. Using this strategy, we successfully isolated one mold strain with superior lipolytic ability for the hydrolysis of monoacylglycerols. The mold was identified and designated as Paecilomyces nostocoides NTU-FC-LP01. The lyophilized mycelia of the isolated mold used as a biocatalyst showed high specificity toward monoacylglycerols rather than di- and tri- acylglycerols. Furthermore, the lyophilized mycelia catalyzed the monoolein synthesis through the direct esterification of oleic acid and glycerol. It indicated that the lyophilized mycelia of the present P. nostocoides NTU-FC-LP01 could be used as a natural immobilized biocatalyst for the glycerol/oleic acid esterification to produce monoolein.
Starting Page	1651
Ending Page	1658
Page Count	8
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	25
Issue Number	9
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-05-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Paecilomyces nostocoides Lipolysis Monoacylglycerols Screening Microbiology Environmental Engineering/Biotechnology Applied Microbiology Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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