NDLI: The response of aggregated Pseudomonas putida CP1 cells to UV-C and UV-A/B disinfection

Content Provider	Springer Nature Link
Author	Maganha de Almeida, Ana C. Quilty, Bríd
Copyright Year	2016
Abstract	UV radiation is a spread method used worldwide for the disinfection of water. However, much of the research on the disinfection of bacterial cells by UV has focused on planktonic cells. Many bacterial cells in nature are present in clumps or aggregates, and these aggregates, which are more resistant to disinfection than their planktonic counterparts, can be problematic in engineered water systems. The current research used Pseudomonas putida (P. putida) CP1, an environmental and non-pathogenic microorganism which autoaggregates when grown under certain conditions, as a model organism to simulate aggregated cells. The study investigated the response of both the planktonic and the aggregated forms of the bacterium to UV-C (λ = 253.7 nm) and UV-A/B (λ > 300 nm) disinfection at laboratory scale in a minimal medium. The planktonic cells of P. putida CP1 were inactivated within 60 s by UV-C and in 60 min by UV-A/B; however, the aggregated cells required 120 min of UV-C treatment and 240 min of UV-A/B radiation to become inactive. The size of the aggregate was reduced following UV treatment. Although all the cells had lost culturability, viability as measured by the LIVE/DEAD$^{®}$ stain and epifluorescence microscopy was not completely lost and the cells all demonstrated regrowth after overnight incubation in the dark.
Starting Page	1
Ending Page	10
Page Count	10
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	32
Issue Number	11
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-09-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Planktonic cells Aggregated cells UV disinfection Culturability Viability Dark repair EPS Applied Microbiology Biotechnology Biochemistry Environmental Engineering/Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The response of Pseudomonas putida CP1 cells to nutritional, chemical and environmental stresses

The Influence of Glucose and Fructose on the Degradation of 2-Chlorophenol by Pseudomonas putida CP1

Responses of unsaturated Pseudomonas putida CZ1 biofilms to environmental stresses in relation to the EPS composition and surface morphology

Pseudomonas putida are environmental reservoirs of antimicrobial resistance to β-lactamic antibiotics

Expression, purification, and characterization of alanine racemase from Pseudomonas putida YZ-26

Impact of UV-B radiation on Clonostachys rosea germination and growth

Characterization of a strain of Pseudomonas putida isolated from agricultural soil that degrades cadusafos (an organophosphorus pesticide)

Efficient hydroxylation of 1,8-cineole with monoterpenoid-resistant recombinant Pseudomonas putida GS1

The effect of preculture conditions on the culturability and viability of Aeromonas hydrophila when exposed to oligotrophic and cold stresses

The response of aggregated Pseudomonas putida CP1 cells to UV-C and UV-A/B disinfection

Similar Documents

The response of Pseudomonas putida CP1 cells to nutritional, chemical and environmental stresses

The Influence of Glucose and Fructose on the Degradation of 2-Chlorophenol by Pseudomonas putida CP1

Responses of unsaturated Pseudomonas putida CZ1 biofilms to environmental stresses in relation to the EPS composition and surface morphology

Pseudomonas putida are environmental reservoirs of antimicrobial resistance to β-lactamic antibiotics

Expression, purification, and characterization of alanine racemase from Pseudomonas putida YZ-26

Impact of UV-B radiation on Clonostachys rosea germination and growth

Characterization of a strain of Pseudomonas putida isolated from agricultural soil that degrades cadusafos (an organophosphorus pesticide)

Efficient hydroxylation of 1,8-cineole with monoterpenoid-resistant recombinant Pseudomonas putida GS1

The effect of preculture conditions on the culturability and viability of Aeromonas hydrophila when exposed to oligotrophic and cold stresses

The response of aggregated Pseudomonas putida CP1 cells to UV-C and UV-A/B disinfection