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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wang, Pin Mei Wu, Xue Chang Chi, Xiao Qin Li, Yu Dong Zheng, Dao Qiong Ding, Rui Min, Hang |
| Copyright Year | 2010 |
| Abstract | To overcome the drawbacks of protoplast fusion in industrial breeding, strain-specific molecular markers were applied to select hybrids of industrial Saccharomyces cerevisiae strains. Random Amplified Polymorphic DNA (RAPD) analysis was used to generate strain-specific RAPD markers for two industrial yeast strains, Z8 and Z9. For industrial and technical controls, two RAPD markers with non-coding regions were converted into stable Sequence Characterized Amplified Region (SCAR) markers. Hybrids of Z8 and Z9 were obtained by protoplast fusion in combination with SCAR markers and were found to increase ethanol production by 4.3–8.1%. Results suggested that protoplast fusion could be combined with RAPD-SCAR molecular markers and applied in industrial breeding instead of auxotrophic markers. |
| Starting Page | 185 |
| Ending Page | 188 |
| Page Count | 4 |
| File Format | |
| ISSN | 09593993 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume Number | 27 |
| Issue Number | 1 |
| e-ISSN | 15730972 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2010-05-14 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Industrial breeding Molecular marker Protoplast fusion Saccharomyces cerevisiae Biotechnology Applied Microbiology Microbiology Environmental Engineering/Biotechnology Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Applied Microbiology and Biotechnology Biotechnology |
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