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| Content Provider | Springer Nature Link |
|---|---|
| Author | Li, Kun tai Peng, Wei fu Xia, Wei Huang, Lin Cheng, Xin |
| Copyright Year | 2015 |
| Abstract | Many investigations have revealed that a certain concentration of osmolality was indispensable for efficient acarbose production, but little information was available on the response mechanism of acarbose-producing strains to osmotic stress. By using the gas chromatography–mass spectrometry (GC–MS) analysis coupled with the enzyme activity determination of central carbon metabolism, the present work investigated the metabolic characteristics of industrial acarbose-producing Actinoplanes sp. A56 under various osmolality levels. Relatively high osmolality (450–500 mOsm/kg) appeared to favor efficient acarbose production by Actinoplanes sp. A56, although it inhibited cell growth. Further GC–MS analysis showed that fatty acids were the uppermost differential intracellular metabolites under various osmolality levels, and the relatively high osmolality resulted in increases in levels of fatty acids. |
| Starting Page | 1 |
| Ending Page | 7 |
| Page Count | 7 |
| File Format | |
| ISSN | 09593993 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume Number | 32 |
| Issue Number | 1 |
| e-ISSN | 15730972 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2015-12-28 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Actinoplanes sp. A56 Acarbose Metabolic response Osmolality level Applied Microbiology Biotechnology Biochemistry Environmental Engineering/Biotechnology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Applied Microbiology and Biotechnology Biotechnology |
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