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| Content Provider | Springer Nature Link |
|---|---|
| Author | Prasad, Shreenath Misra, Anurag Jangir, Ved Prakash Awasthi, Abhishek Raj, Jog Bhalla, Tek Chand |
| Copyright Year | 2006 |
| Abstract | Rhodococcus sp. NDB 1165, a nitrile-transforming organism was isolated from temperate forest soil of Himalayas. The nitrilase (EC 3.5.5.2) activity of this organism had higher substrate specificity toward aromatic nitriles (benzonitrile, 3-cyanopyridine and 4-cyanopyridine) and unsaturated aliphatic nitrile (acrylonitrile) in comparison to saturated aliphatic nitriles (acetonitrile, propionitrile, butyronitrile and isobutyronitrile) nitrile and arylacetonitrile (phenylacetonitrile and indole-3-acetonitrile). The nitrilase of Rhodococcus sp. NDB 1165 was inducible in nature and propionitrile proved to be an efficient inducer. However, the salts of ferrous and cobalt ions had an inhibitory effect. Under optimized reaction conditions (pH 8.0 and temperature 45°C) the nitrilase activity of this organism was 2.39 ± 0.07 U/mg dry cell mass (dcm). The half-life of this enzyme was 150 min and 40 min at 45°C and 50°C respectively. However, it was quite stable at 40°C and around 58 % activity was retained even after 6 h at this temperature. The V $_{max}$ and K $_{m}$ value of this nitrilase were 1.67 µmol/ml min and 0.1 M respectively using 3-cyanopyridine as substrate. However, the decrease in V $_{max}$ and K $_{m}$ values (0.56 µmol/ml min and 0.02 M, respectively) were ␣observed at >0.05 M 3-cyanopyridine which revealed that this enzyme experienced uncompetitive inhibition at higher substrate concentrations. Under optimized reaction conditions, 1.6 M 3-cyanopyridine was successfully converted in to nicotinic acid using 2.0 mg resting cells (dcm)/ml reaction mixture in 11 h. This is the highest production of nicotinic acid i.e. 8.95 mg/mg resting cells (dcm)/h as compared to nitrilase systems reported hitherto. |
| Starting Page | 345 |
| Ending Page | 353 |
| Page Count | 9 |
| File Format | |
| ISSN | 09593993 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume Number | 23 |
| Issue Number | 3 |
| e-ISSN | 15730972 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2006-07-20 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | 3-Cyanopyridine Fed-batch reaction Nicotinic acid Nitrilase Rhodococcus sp. NDB 1165 Microbiology Environmental Biotechnology Biochemistry Biotechnology Applied Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Applied Microbiology and Biotechnology Biotechnology |
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