NDLI: Kinetic basis of celite (CM 2:1) addition on the biosynthesis of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) by Aspergillus oryzae ME$_{2}$ using l-tyrosine as a basal substrate

Please wait, while we are loading the content...

Kinetic basis of celite (CM 2:1) addition on the biosynthesis of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) by Aspergillus oryzae ME$_{2}$ using l-tyrosine as a basal substrate

Purification and characterization of a sodium-stimulated β-galactosidase from Bifidobacterium longum CCRC 15708

Enhancement of gene detection frequencies by combining DNA-based stable-isotope probing with the construction of metagenomic DNA libraries

Proteomics of a plant growth-promoting rhizobacterium, Pseudomonas fluorescens MSP-393, subjected to salt shock

Production of Extracellular Halo-alkaline Protease from a Newly Isolated Haloalkaliphilic Bacillus sp. Isolated from Seawater in Western India

Chemical, microbiological and plant analysis of soil fertilized with alkaline hydrolysate of sheep’s wool waste

Effect of nitrogen source on lignocellulolytic enzyme production by white-rot basidiomycetes under solid-state cultivation

Analysis of metabolic fluxes for hyaluronic acid (HA) production by Streptococcus zooepidemicus

Cloning of Lactobacillus plantarum IAM 12477 lysine biosynthetic genes encoding functional aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase

World Journal of Microbiology and Biotechnology : Volume 22, Issue 3, March 2006

World Journal of Microbiology and Biotechnology : Volume 22, Issue 2, February 2006

World Journal of Microbiology and Biotechnology : Volume 22, Issue 1, January 2006

World Journal of Microbiology and Biotechnology : Volume 21

World Journal of Microbiology and Biotechnology : Volume 20

World Journal of Microbiology and Biotechnology : Volume 19

World Journal of Microbiology and Biotechnology : Volume 18

World Journal of Microbiology and Biotechnology : Volume 17

World Journal of Microbiology and Biotechnology : Volume 16

World Journal of Microbiology and Biotechnology : Volume 15

World Journal of Microbiology and Biotechnology : Volume 14

World Journal of Microbiology and Biotechnology : Volume 13

Kinetic basis of celite (CM 2:1) addition on the biosynthesis of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) by Aspergillus oryzae ME$_{2}$ using l-tyrosine as a basal substrate

Content Provider	Springer Nature Link
Author	Ali, Sikander
Copyright Year	2005
Abstract	Celite is a unique variety of 2:1 clay mineral and is referred to as hydrous mica because it is believed to be the␣weathering product of mica. The present study describe the effect of celite on microbiological conversion of l-tyrosine to 3,4-dihydroxyphenyl-l-alanine (l-DOPA) by Aspergillus oryzae ME$_{2}$. At optimum pH (pH$_{o}$=6), and temperature (t=30 °C), 100% sugars were utilized for cell mass formation. Mould mycelium was used for biochemical conversion of l-tyrosine to l-DOPA because tyrosinases, β-carboxylases and tyrosine hydroxylases are intracellular enzymes. The maximum conversion of l-tyrosine to l-DOPA (0.428 mg/ml) was achieved after 60 min of reaction. To further enhance the production of l-DOPA, different concentration of celite were added to the reaction mixture. Best results were observed when the concentration of celite was 3.5 μM (1.686 mg l-DOPA produced/ml with 1.525 mg l-tyrosine consumed/ml). There was up to 3-fold enhancement in the product formation rate. This enhancement is the highest so far reported. Celite not only increased enzyme activity but also enhanced the permeability of the cell membrane to facilitate the secretion of enzymes in to the reaction broth. The comparison of kinetic parameters showed the ability of the mutant to yield l-DOPA (i.e., Y $_{p/x}$ 7.360±0.04 mg/mg). When the culture grown on various celite levels was monitored for Q $_{p}$, Q $_{s}$ and q $_{p}$, there was significant enhancement (P<0.025) in these variables over the control, which indicates that the study can be commercially applicable (HS, LSD 0.456).
Starting Page	347
Ending Page	353
Page Count	7
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	22
Issue Number	4
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2005-10-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	A spergillus oryzae Biochemical conversion Celite l-DOPA l-tyrosine Parkinson’s disease Microbiology Environmental Engineering/Biotechnology Biochemistry Biotechnology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Microbiological Transformation of L-Tyrosine to 3,4-Dihydroxyphenyl L-Alanine (L-Dopa) by a Mutant Strain of Aspergillus oryzae UV-7

Innovative Effect of Illite on Improved Microbiological Conversion of L-Tyrosine to 3,4 Dihydroxy Phenyl L-Alanine (L-DOPA) by Aspergillus oryzae ME2 Under Acidic Reaction Conditions

Hyperproduction of 3,4-Dihydroxyphenyl-L-alanine (L-Dopa) Using Erwinia herbicola Cells Carrying a Mutant Transcriptional Regulator TyrR

Production ofL-DOPA(3,4-dihydroxyphenyl-L-alanine) from benzene by using a hybrid pathway

Mutation of Aspergillus oryzae for improved production of 3, 4-dihydroxy phenyl-L-alanine (L-DOPA) from L-tyrosine

Mutation of Aspergillus oryzae for improved production of 3, 4-dihydroxy phenyl-L-alanine (L-DOPA) from L-tyrosine

Inducive effect of cresoquinone on microbiological transformation of L-tyrosine to 3,4 dihydroxy phenyl L-alanine by Aspergillus oryzae NG-11P1

Behavior of fluorinated analogs of L-(3,4-dihydroxyphenyl)alanine and L-threo-(3,4-dihydroxyphenyl)serine as substrates for Dopa decarboxylase.

L-Ascorbate, a strong inducer of L-dopa (3,4-dihydroxy-L-phenylalanine) production from pre-grown mycelia of Aspergillus oryzae NRRL-1560.

Kinetic basis of celite (CM 2:1) addition on the biosynthesis of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) by Aspergillus oryzae ME$_{2}$ using l-tyrosine as a basal substrate

Similar Documents

Microbiological Transformation of L-Tyrosine to 3,4-Dihydroxyphenyl L-Alanine (L-Dopa) by a Mutant Strain of Aspergillus oryzae UV-7

Innovative Effect of Illite on Improved Microbiological Conversion of L-Tyrosine to 3,4 Dihydroxy Phenyl L-Alanine (L-DOPA) by Aspergillus oryzae ME2 Under Acidic Reaction Conditions

Hyperproduction of 3,4-Dihydroxyphenyl-L-alanine (L-Dopa) Using Erwinia herbicola Cells Carrying a Mutant Transcriptional Regulator TyrR

Production ofL-DOPA(3,4-dihydroxyphenyl-L-alanine) from benzene by using a hybrid pathway

Mutation of Aspergillus oryzae for improved production of 3, 4-dihydroxy phenyl-L-alanine (L-DOPA) from L-tyrosine

Mutation of Aspergillus oryzae for improved production of 3, 4-dihydroxy phenyl-L-alanine (L-DOPA) from L-tyrosine

Inducive effect of cresoquinone on microbiological transformation of L-tyrosine to 3,4 dihydroxy phenyl L-alanine by Aspergillus oryzae NG-11P1

Behavior of fluorinated analogs of L-(3,4-dihydroxyphenyl)alanine and L-threo-(3,4-dihydroxyphenyl)serine as substrates for Dopa decarboxylase.

L-Ascorbate, a strong inducer of L-dopa (3,4-dihydroxy-L-phenylalanine) production from pre-grown mycelia of Aspergillus oryzae NRRL-1560.

Kinetic basis of celite (CM 2:1) addition on the biosynthesis of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) by Aspergillus oryzae ME$_{2}$ using l-tyrosine as a basal substrate