NDLI: Effects of chitin binding domain on enzymatic properties and insecticidal activity of Bombyx mori chitinase

Content Provider	Springer Nature Link
Author	Fan, Yanhua Guo, Shujun Pei, Xiaoqiong Zhang, Yongjun Luo, Zhibing Pei, Yan
Copyright Year	2010
Abstract	Bombyx mori chitinase (Bmchi) possesses a catalytic domain and a cysteine-rich C-terminal domain. Wild-type and a C-terminal truncated form (Bmchi∆c) were expressed and purified from recombinant Pichia pastoris hosts. Loss of the C-teminal domain decreased the ability of the chitinase to bind and degrade insoluble substrates. Differences in optimal pH and temperature, thermostability, and ability to degrade certain oligosaccharide substrates were also observed between Bmchi and Bmchi∆c. To determine whether these proteins could be used to increase virulence of entomopathogenic fungi, Bmchi and Bmchi∆c were introduced into Beauveira bassiana under control of the A. nidulans gpdA constitutive promoter. Insect bioassays using WT and transformed B. bassiana strains revealed expression of Bmchi significantly improved fungal virulence compared to the WT parental strain, whereas expression of Bmchi∆c in B. bassiana had only a nominal effect. These results indicate that the B. mori chitinase can be used to improved fungal efficiency but that the C-terminal domain is essential for this function.
Starting Page	1551
Ending Page	1558
Page Count	8
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	27
Issue Number	7
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-11-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bombyx mori Chitinase Ecdysis Virulence Chitin binding domain (ChBD) Biotechnology Microbiology Biochemistry Environmental Engineering/Biotechnology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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