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| Content Provider | Springer Nature Link |
|---|---|
| Author | Torres, M. J. Petroselli, G. Daz, M. Erra Balsells, R. Audisio, M. C. |
| Copyright Year | 2015 |
| Abstract | In this work a new Bacillus sp. strain, isolated from honey, was characterized phylogenetically. Its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Bacillus CBMDC3f was phylogenetically characterized as Bacillus subtilis subsp. subtilis after rRNA analysis of the 16S subunit and the gyrA gene (access codes Genbank JX120508 and JX120516, respectively). Its antibacterial potential was evaluated against Listeria monocytogenes (9 strains), B. cereus (3 strains) and Staphylococcus aureus ATCC29213. Its cell suspension and cell-free supernatant (CFS) exerted significant anti-Listeria and anti-S. aureus activities, while the lipopeptides fraction (LF) also showed anti-B. cereus effect. The UV-MALDI-MS analysis revealed surfactin, iturin and fengycin in the CFS, whereas surfactin predominated in the LF. The CFS from CBMDC3f contained surfactin, iturin and fengycin with four, two and four homologues per family, respectively, whereas four surfactin, one iturin and one fengycin homologues were identified in the LF. For some surfactin homologues, their UV-MALDI-TOF/TOF (MS/MS; Laser Induced Decomposition method, LID) spectra were also obtained. Mass spectrometry analysis contributed with relevant information about the type of lipopeptides that Bacillus strains can synthesize. From our results, surfactin would be the main metabolite responsible for the antibacterial effect. |
| Starting Page | 929 |
| Ending Page | 940 |
| Page Count | 12 |
| File Format | |
| ISSN | 09593993 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume Number | 31 |
| Issue Number | 6 |
| e-ISSN | 15730972 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2015-03-29 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacillus subtilis subsp. subtilis Foodborne pathogens Lipopeptide homologues UV-MALDI-TOF MS Surfactin Applied Microbiology Biotechnology Biochemistry Environmental Engineering/Biotechnology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Applied Microbiology and Biotechnology Biotechnology |
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