NDLI: Identification of bacterial species on Lippia multiflora herbal tea leaves and the influence of steam pasteurization

Content Provider	Springer Nature Link
Author	Arthur, Hanson Cameron, Michelle Joubert, Elizabeth Witthuhn, R. Corli
Copyright Year	2010
Abstract	The consumption of herbal teas is an increasing phenomenon among tea consumers globally. Some of these herbal teas are not pre-treated to reduce their microbial load before consumption, and thus constitute a health risk to consumers. In this study, the effect of steam pasteurization, at >99 °C for 2.5 min, on the microbial load of Lippia multiflora herbal tea leaves was evaluated. Microbial enumeration was conducted on potato dextrose agar, plate count agar, violet red bile agar, yeast peptone dextrose agar, and DeMan-Rogosa-Sharpe agar. Morphologically distinct colonies were isolated, sub-cultured and their Gram reaction recorded. These bacteria were identified to the species level using 16S ribosomal DNA sequence data. Most of the bacteria identified belonged to the genus Bacillus. One species each from the genera Pantoea and Kocuria was also identified, but only the Bacillus species survived the steam pasteurization treatment. Coliform bacteria detected prior to pasteurization were not detected after the steam treatment. Steam pasteurization reduced the microbial load from 10$^{4}$ to 10$^{2 }$c.f.u.g$^{−1}$and it is potentially an effective method to treat L. multiflora herbal teas prior to consumption. It is important to note that the steam treatment should complement good agricultural and hygienic practices rather than replace them, as some bacteria can survive this treatment.
Starting Page	443
Ending Page	447
Page Count	5
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	27
Issue Number	2
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-05-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lippia multiflora Herbal tea Microbial load Steam pasteurization Microbiology Environmental Engineering/Biotechnology Applied Microbiology Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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