NDLI: Daunorubicin forms a specific complex with a secreted serine protease of Streptomyces peucetius

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Daunorubicin forms a specific complex with a secreted serine protease of Streptomyces peucetius

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Daunorubicin forms a specific complex with a secreted serine protease of Streptomyces peucetius

Content Provider	Springer Nature Link
Author	Dubey, Rashmi Kattusamy, Karuppasamy Dharmalingam, Kuppamuthu Prasad, Ranjan
Copyright Year	2013
Abstract	Daunorubicin forms specific complex with an extracellular protease in the Streptomyces peucetius culture. The drug-protein complex co-migrates in non-denaturing PAGE as a red band. De novo peptide sequencing by nano-LC–ESI–MS/MS and MASCOT analysis identified the daunorubicin binding protein as serine protease precursor. The same protease precursor was purified sans the daunorubicin, from the mutant named ΔDPSAmut, which is deficient in daunorubicin production. Daunorubicin was added to ΔDPSAmut culture and the protease readily formed the daunorubicin-protease complex. Ability of serine protease precursor to form a selective complex with daunorubicin was confirmed by this study. Selective binding of protease to daunorubicin was seen as self-resistance determinant for the organism to survive toxic levels of the drug outside the cell. Daunorubicin-protease complex placed on S. peucetius lawn did not produce clearing zone around it, whereas daunorubicin purified from the complex did produce the clearing zone. Thereby it is concluded that the protease sequesters daunorubicin to prevent its entry into cells. Sequestration of daunorubicin by extracellular protease helps the organism to maintain a steady state sub-inhibitory level of drug around the cells. A new self-resistance determinant is reported here.
Starting Page	253
Ending Page	261
Page Count	9
File Format	PDF
ISSN	09593993
Journal	World Journal of Microbiology and Biotechnology
Volume Number	30
Issue Number	1
e-ISSN	15730972
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-07-31
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Streptomyces peucetius Daunorubicin Serine protease Self-resistance Applied Microbiology Biotechnology Biochemistry Environmental Engineering/Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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Daunorubicin forms a specific complex with a secreted serine protease of Streptomyces peucetius.

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Daunorubicin forms a specific complex with a secreted serine protease of Streptomyces peucetius

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