NDLI: Occurrence of Dehalococcoides and Reductive Dehalogenase Genes in Microcosms, a Constructed Wetland and Groundwater from a Chlorinated Ethene Contaminated Field Site as Indicators for In Situ Reductive Dehalogenation

Content Provider	Springer Nature Link
Author	Mészáros, Éva Imfeld, Gwenaël Nikolausz, Marcell Nijenhuis, Ivonne
Copyright Year	2013
Abstract	Thus far, members of the genus Dehalococcoides are the only microorganisms known to dehalogenate chlorinated ethenes to ethene and thereby detoxify these common groundwater pollutants. Therefore, it is important to characterize the taxonomic and functional diversity of these key microorganisms and their reductive dehalogenase (RDase) genes in contaminated aquifers for assessing the natural attenuation potential. Little is known about the diversity of RDase genes under field conditions or in laboratory systems under selective pressure during dechlorination activities. Here, we evaluate the diversity of Dehalococcoides sp. and three RDase genes in groundwater as well as in water from a constructed wetland and microcosms setup with contaminated groundwater from the same field site in Bitterfeld (Saxony-Anhalt, Germany). The presence and relative abundance of Pinellas and Cornell subgroups of Dehalococcoides was evaluated by a novel direct sequencing method, which revealed that all sequences were identical and affiliated to the Pinellas subgroup. Contrarily, our results showed remarkable differences at the functional gene level between the systems. Of the vinyl chloride reductase genes, vcrA was detected in samples from the groundwater, wetland, and microcosms, whereas bvcA was only found in wetland and microcosm samples. The trichloroethene dehalogenase gene, tceA could not be detected at all, although complete dehalogenation activity of higher chlorinated ethenes was observed. Our study demonstrates that although the Dehalococcoides 16S rRNA gene sequences retrieved from the investigated systems were identical, the RDase gene diversity varied among the systems, according to the spectrum of the chlorinated ethenes present.
Starting Page	1
Ending Page	12
Page Count	12
File Format	PDF
ISSN	00496979
Journal	Water, Air, and Soil Pollution
Volume Number	224
Issue Number	11
e-ISSN	15732932
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-10-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	RDase genes Reductive dehalogenation Chlorinated ethenes Bitterfeld Direct sequencing Environment Atmospheric Protection/Air Quality Control/Air Pollution Waste Water Technology Water Pollution Control Water Management Aquatic Pollution Soil Science & Conservation Hydrogeology Climate Change
Content Type	Text
Resource Type	Article
Subject	Ecological Modeling Environmental Chemistry Pollution Environmental Engineering Water Science and Technology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Reductive Dehalogenase Gene Expression as a Biomarker for Physiological Activity of Dehalococcoides spp. (2006)

Reductive dechlorination of chlorinated ethenes and 1,2-dichloroethene by “dehalococcoides ethenogenes (1999).

Reductive dehalogenase gene expression as a biomarker for physiological activity of Dehalococcoides spp (2006)

Effects of tidal operation on pilot-scale horizontal subsurface flow constructed wetland treating sulfate rich wastewater contaminated by chlorinated hydrocarbons

Dual Element (C/Cl) Isotope Analysis Indicates Distinct Mechanisms of Reductive Dehalogenation of Chlorinated Ethenes and Dichloroethane in Dehalococcoides mccartyi Strain BTF08 With Defined Reductive Dehalogenase Inventories

Quantifying genes and transcripts to assess the in situ physiology of “Dehalococcoides” spp. in a trichloroethene-contaminated groundwater site (2008)

Abiotic degradation of chlorinated ethanes and ethenes in water

Chloroethene degradation and expression of Dehalococcoides dehalogenase genes in cultures originating from Yangtze sediments

Use of a reactive transport model to describe reductive dechlorination (RD) as a remediation design tool: application at a CAH-contaminated site

Occurrence of Dehalococcoides and Reductive Dehalogenase Genes in Microcosms, a Constructed Wetland and Groundwater from a Chlorinated Ethene Contaminated Field Site as Indicators for In Situ Reductive Dehalogenation

Similar Documents

Reductive Dehalogenase Gene Expression as a Biomarker for Physiological Activity of Dehalococcoides spp. (2006)

Reductive dechlorination of chlorinated ethenes and 1,2-dichloroethene by “dehalococcoides ethenogenes (1999).

Reductive dehalogenase gene expression as a biomarker for physiological activity of Dehalococcoides spp (2006)

Effects of tidal operation on pilot-scale horizontal subsurface flow constructed wetland treating sulfate rich wastewater contaminated by chlorinated hydrocarbons

Dual Element (C/Cl) Isotope Analysis Indicates Distinct Mechanisms of Reductive Dehalogenation of Chlorinated Ethenes and Dichloroethane in Dehalococcoides mccartyi Strain BTF08 With Defined Reductive Dehalogenase Inventories

Quantifying genes and transcripts to assess the in situ physiology of “Dehalococcoides” spp. in a trichloroethene-contaminated groundwater site (2008)

Abiotic degradation of chlorinated ethanes and ethenes in water

Chloroethene degradation and expression of Dehalococcoides dehalogenase genes in cultures originating from Yangtze sediments

Use of a reactive transport model to describe reductive dechlorination (RD) as a remediation design tool: application at a CAH-contaminated site

Occurrence of Dehalococcoides and Reductive Dehalogenase Genes in Microcosms, a Constructed Wetland and Groundwater from a Chlorinated Ethene Contaminated Field Site as Indicators for In Situ Reductive Dehalogenation