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| Content Provider | Springer Nature Link |
|---|---|
| Author | Behzadian, Farida Sabahi, Farzaneh Karimi, Mohsen Sadeghizadeh, Majid Maghsoudi, Nader Forooshani, Ramin Sarrami Shahinsaz, Leila |
| Copyright Year | 2005 |
| Abstract | Hepatitis D (delta) virus (HDV) is a subviral pathogen agent and a satellite of Hepatitis B virus. Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, the entire nucleotide sequence of an HDV isolate from an Iranian patient (IR-1) was obtained using twelve pairs of primers to amplify six overlapping fragments covering the whole HDV genome by RT-nested PCR. Phylogenetic and pairwise alignments were done on this new isolate to determine IR-1 position among other isolates. Our results indicate that IR-1 contains 1676 nucleotides encoding 214 a.a. of the hepatitis delta antigen (HDAg). This new isolate belongs to genotype I with most sequence similarity to an Italian HDV isolate (92.6%). At amino acid level, predicted HDAg sequence of IR-1 revealed the most homology with those of Italian and Lebanese isolates. Data analysis confirmed genetic variability and heterogeneity of the HDV species isolated from different geographical areas. |
| Starting Page | 383 |
| Ending Page | 393 |
| Page Count | 11 |
| File Format | |
| ISSN | 09208569 |
| Journal | Virus Genes |
| Volume Number | 30 |
| Issue Number | 3 |
| e-ISSN | 1572994X |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2005-01-01 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | alignment complete genome sequence hepatitis D virus Iranian phylogenetic analysis RT-PCR Medical Microbiology Virology Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Genetics Medicine Molecular Biology |
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