NDLI: Complete nucleotide sequence of Klebsiella phage P13 and prediction of an EPS depolymerase gene

Content Provider	Springer Nature Link
Author	Shang, Anqi Liu, Yang Wang, Jianlei Mo, Zhaolan Li, Guiyang Mou, Haijin
Copyright Year	2014
Abstract	The complete genome of Klebsiella phage P13 was sequenced and analyzed. Bacteriophage P13 has a double-stranded linear DNA with a length of 45,976 bp and a G+C content of 51.7 %, which is slightly lower than that of Klebsiella pneumoniae KCTC 2242. The codon biases of phage P13 are very similar to those of SP6-like phages and K. pneumoniae KCTC 2242. Bioinformatics analysis shows that the phage P13 genome has 282 open reading frames (ORFs) that are greater than 100 bp in length, and 50 of these ORFs were identified as predicted genes with an average length of 833 bp. Among these genes, 41 show homology to known proteins in the GenBank database. The functions of the 24 putative proteins were investigated, and 13 of these were found to be highly conserved. According to the homology analysis of the 50 predicted genes and the whole genome, phage P13 is homologous to SP6-like phages. Furthermore, the morphological characteristics of phage P13 suggest that it belongs to the SP6-like viral genus of the Podoviridae subfamily Autographivirinae. Two hypothetical genes encoding an extracellular polysaccharide depolymerase were predicted using PSI-BLAST. This analysis serves as groundwork for further research and application of the enzyme.
Starting Page	118
Ending Page	128
Page Count	11
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	50
Issue Number	1
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2014-11-13
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Klebsiella phage P13 Complete genome sequencing Predicted genes Genomic comparison EPS depolymerase Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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