NDLI: Three gene products of a begomovirus–betasatellite complex restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana

Content Provider	Springer Nature Link
Author	Saeed, Muhammad Krczal, Gabi Wassenegger, Michael
Copyright Year	2014
Abstract	Single-stranded DNA geminiviruses replicate via double-stranded DNA intermediates forming mini-chromosomes that are targets for transcriptional gene silencing (TGS) in plants. The ability of the cotton leaf curl Kokhran virus (CLCuKoV)–cotton leaf curl Multan betasatellite (CLCuMuB) proteins, replication-associated protein (Rep), transcriptional activator protein (TrAP), C4, V2 and βC1, to suppress TGS was investigated by using the Nicotiana benthamiana line 16-TGS (16-TGS) harbouring a transcriptionally silenced green fluorescent protein (GFP) transgene. Inoculation of 16-TGS plants with a recombinant potato virus X vector carrying Rep, TrAP or βC1 resulted in re-expression of GFP. Northern blot analysis confirmed that the observed GFP fluorescence was associated with GFP mRNA accumulation. These results indicated that Rep, TrAP and βC1 proteins of CLCuKoV–CLCuMuB can re-activate the expression of a transcriptionally silenced GFP transgene in N. benthamiana. Although Rep, TrAP, or βC1 proteins have, for other begomoviruses or begomoviruses–betasatellites, been previously shown to have TGS suppressor activity, this is the first report demonstrating that a single begomovirus–betasatellite complex encodes three suppressors of TGS.
Starting Page	340
Ending Page	344
Page Count	5
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	50
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2014-12-24
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Begomovirus Betasatellite Transcriptional gene silencing Methylation Host defence Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana.

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Three gene products of a begomovirus–betasatellite complex restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana

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