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| Content Provider | Springer Nature Link |
|---|---|
| Author | Shimode, Sayumi Yoshikawa, Rokusuke Hoshi, Shigeki Nakaya, Yuki Sakaguchi, Shoichi Kobayashi, Takeshi Miyazawa, Takayuki |
| Copyright Year | 2012 |
| Abstract | RD-114 virus is a replication-competent feline endogenous retrovirus. RD-114 virus contaminates several feline and canine live attenuated vaccines and the issue of contamination of RD-114 virus in vaccines should be solved. To date, three infectious molecular clones (pSc3c, pCRT1, and pRD-UCL) have been reported. In this study, we sequenced the entire nucleotide sequence of pRD-UCL and compared the nucleotide sequences of the three infectious molecular clones. As a result, these three infectious clones were nearly identical with each other in gag-pol and env coding regions. These data support the notion that the active locus of infectious RD-114 virus is single in the feline genome. The length of long terminal repeat (LTR) of pCRT1 was 47 bp shorter than those of pSc3c and pRD-UCL. The 47-bp sequence named direct repeat A (DR-A) was duplicated in the U3 region in pSc3c and pRD-UCL. Although several potential enhancer binding sites are present in the DR-A, there was no significant difference in promoter activities between the LTRs of pRD-UCL and pCRT1 in two human cell lines. We also analyzed the splicing pattern of the RD-114 virus by reverse transcription-polymerase chain reaction and confirmed that RD-114 virus is a simple retrovirus. The data presented here will provide basic information about RD-114 virus to solve the contamination issue in live attenuated vaccines. |
| Starting Page | 393 |
| Ending Page | 397 |
| Page Count | 5 |
| File Format | |
| ISSN | 09208569 |
| Journal | Virus Genes |
| Volume Number | 45 |
| Issue Number | 2 |
| e-ISSN | 1572994X |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2012-05-26 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cats RD-114 virus Infectious molecular clone Full genome sequencing Splicing pattern Medical Microbiology Virology Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Genetics Medicine Molecular Biology |
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