NDLI: Comparative analysis of budded virus infectivity of Bombyx mandarina and B. mori nucleopolyhedroviruses

Content Provider	Springer Nature Link
Author	Kokusho, Ryuhei Zhang, Chuan Xi Shimada, Toru Katsuma, Susumu
Copyright Year	2011
Abstract	Bombyx mandarina nucleopolyhedrovirus (BomaNPV) is a variant of Bombyx mori nucleopolyhedrovirus (BmNPV). BomaNPV S1 strain has been reported to be significantly less virulent than the BmNPV T3 strain via the oral infection route in B. mori larvae, but other features of S1 including budded virus (BV) infectivity and virus propagation in cultured cells are still unknown. In this study, we compared BV infectivity of S1 and T3 in B. mori larvae and cultured cells. Larval bioassays by intrahemocoelic BV injection revealed that the median lethal dose of S1’s BV was approximately three times lower than that of T3. In addition, S1 produced more BVs and occlusion bodies (OBs) in the hemolymph of B. mori larvae compared with T3. Furthermore, we observed that the locomotion was enhanced earlier and the median lethal time was shorter in S1-infected larvae compared with those in T3-infected larvae. Western blot analysis of S1- and T3-infected BmN cells revealed that expression of late and very late gene products in S1-infected cells was higher than that in T3-infected cells. Collectively, these results clearly show that S1’s BV infectivity is higher than that of T3 in both B. mori larvae and cultured cells, although S1’s OBs are much less infectious to B. mori larvae than T3’s.
Starting Page	313
Ending Page	317
Page Count	5
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	43
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2011-05-22
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Baculovirus BomaNPV BmNPV Infectivity BmN Bombyx mori Virology Plant Sciences Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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