NDLI: Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China

Content Provider	Springer Nature Link
Author	Cheng, Yuening Wang, Jianke Zhang, Miao Zhao, Jianjun Shao, Xiqun Ma, Zengjun Zhao, Hang Lin, Peng Wu, Hua
Copyright Year	2015
Abstract	Canine distemper virus (CDV) is a major pathogen not only in raccoon dogs but also in a variety of carnivorous animals, including domesticated animals, particularly if they have not been vaccinated. In this study, a wild-type strain of CDV was isolated from lung tissue from a raccoon dog kept at a fur farm in Jilin Province, China. Cytopathic effects typical of CDV infection were observed after three blind passages in Vero cells, yielding a virus titer of 10$^{4.6}$ TCID$_{50}$/mL. Virus identification was carried out by RT-PCR, immunofluorescence, electron microscopy, and genome sequencing. The results showed that the isolated virus, termed the SY strain, corresponded to the Asia-1 genotype of CDV and has a genome of 15,690 nucleotides. This represents the first complete nucleotide sequence of a CDV strain circulating in raccoon dogs in China.
Starting Page	298
Ending Page	301
Page Count	4
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	51
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2015-08-12
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Canine distemper virus Raccoon dog Genome sequence H gene Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China