NDLI: Genome Prediction of Putative Genome-Linked Viral Protein (VPg) of Astroviruses

Content Provider	Springer Nature Link
Author	Al Mutairy, Badr Walter, Jolan E. Pothen, Alex Mitchell, Douglas K.
Copyright Year	2005
Abstract	Positive-sense single-stranded RNA (+ssRNA) viruses replicate by uncoating the RNA genome for translation to provide viral proteins essential for genome replication and the production of new viral particles. The viral proteins are synthesized from a polyprotein precursor, which is cleaved nascently. The synthesized proteins include viral RNA-dependent RNA polymerase (RdRP), viral genome-linked protein (VPg), and a helicase. VPg is covalently attached to the genomic form of +ssRNA viruses. Helicases and NTPase unwind the RNA before replication. VPg and helicases have been identified in +ssRNA families, however, the presence of VPg and helicase in the Astroviridae, another +ssRNA family, has not been fully elucidated. Computational tools were utilized to provide sequence analysis evidence for the presence and genomic location of astrovirus VPg and helicase. HMMER program v2.1.1 was used to build Hidden Markov Model (HMM) profile for calicivirus VPg to search for conserved motifs in the astrovirus genome. We performed phylogenetic analysis of two genomic regions of astroviruses and caliciviruses (encoding the RdRP and VPg). We identified a putative VPg coding region in astrovirus. This region was located in open reading frame 1a (ORF1 a) and included sites with high sequence similarity to the VPg coding regions of Caliciviridae, Piconaviridae, and Potyviridae. A region encoding a putative astrovirus helicase identified conserved motifs only with pestivirus helicase sequences. Sequence analysis and comparison to other +ssRNA viruses supports the presence of VPg in the Astroviridae. Further laboratory analysis will be necessary to confirm these findings.
Starting Page	21
Ending Page	30
Page Count	10
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	31
Issue Number	1
e-ISSN	1572994X
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	astrovirus genome RNA virus sequence analysis Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The Genome-linked Protein (VPg) of Southern Bean Mosaic Virus is Encoded by the ORF2

Complete Sequence of the RNA Genome of Pneumonia Virus of Mice (PVM)

Phylogenetic Analysis of Turkey Astroviruses Reveals Evidence of Recombination

Complete genomic sequence of Dracaena mottle virus, a distinct badnavirus

A double-stranded RNA as the genome of a potential virus infecting Vicia faba

Molecular detection of novel astroviruses in wild and laboratory mice

Complete genome sequence of an Argentinean isolate of Solenopsis invicta virus 3

Complete nucleotide sequence of Nootka lupine vein-clearing virus

VPg of Potato virus A alone does not suppress RNA silencing but affects virulence of a heterologous virus

Genome Prediction of Putative Genome-Linked Viral Protein (VPg) of Astroviruses

Similar Documents

The Genome-linked Protein (VPg) of Southern Bean Mosaic Virus is Encoded by the ORF2

Complete Sequence of the RNA Genome of Pneumonia Virus of Mice (PVM)

Phylogenetic Analysis of Turkey Astroviruses Reveals Evidence of Recombination

Complete genomic sequence of Dracaena mottle virus, a distinct badnavirus

A double-stranded RNA as the genome of a potential virus infecting Vicia faba

Molecular detection of novel astroviruses in wild and laboratory mice

Complete genome sequence of an Argentinean isolate of Solenopsis invicta virus 3

Complete nucleotide sequence of Nootka lupine vein-clearing virus

VPg of Potato virus A alone does not suppress RNA silencing but affects virulence of a heterologous virus

Genome Prediction of Putative Genome-Linked Viral Protein (VPg) of Astroviruses