NDLI: Expression patterns of prion protein gene in differential genotypes sheep: quantification using molecular beacon real-time RT-PCR

Content Provider	Springer Nature Link
Author	Wang, Chuan Wu, Run Li, Fa Di Liu, Lei Zhang, Xiao Li Zhao, Chun Lin Diao, Xiao Long Guan, Hong Wei
Copyright Year	2011
Abstract	Determination of the transcription level of cellular prion protein (PrP$^{C}$) is essential for understanding its role in organisms and revealing mechanism of susceptibility and resistance to scrapie. However, the expression of prion protein (PrP) mRNA in sheep has not been quantified in great detail in digestive tract which is important during scrapie spread through oral route. Herein, we report on measurement of sheep PrP mRNA using absolute quantitative real-time RT-PCR. Total RNA was isolated from five different regions of the central nervous system (CNS), four regions of lymphoid system, eleven regions of digestive tract, and two reproductive organ tissues of eight sheep of two different genotypes (ARR/ARQ and ARH/ARQ) and PrP mRNA was quantified by real-time RT-PCR using molecular beacon. The results showed that highest levels of PrP mRNA were expressed in thalamus and cerebrum (P < 0.01) of CNS examined, followed by cerebellum, spinal cord, and brain stem. In peripheral organs examined, lymph tissue showed moderate level of PrP expression similar to that in digestive tract and reproduction organs. PrP expression levels in the same tissue of different genotype sheep had significant variation. Our study provided the first detail, tissue-specific and genotype-specific data of PrP mRNA expression in sheep for further studies of pathogenesis of prion diseases.
Starting Page	457
Ending Page	462
Page Count	6
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	42
Issue Number	3
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2011-02-12
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	PrP Sheep mRNA expression Real-time quantitative RT-PCR Molecular beacon ARR/ARQ ARH/ARQ Virology Plant Sciences Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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