NDLI: Tick-borne encephalitis virus: reference strain Sofjin and problem of its authenticity

Content Provider	Springer Nature Link
Author	Kovalev, S. Y. Mukhacheva, T. A. Kokorev, V. S. Belyaeva, I. V.
Copyright Year	2011
Abstract	The Sofjin strain is one of the first isolates of tick-borne encephalitis virus and, due to its wide distribution in virus collections, it has become the reference strain. Until now, GenBank has recorded several sequences associated with the Sofjin strain that have significant differences between each other. We have sequenced the complete genome of the Sofjin strain from a virus collection and a genome fragment of the two vaccine Sofjin strains. According to phylogenetic analysis, we concluded that the GenBank sequences belong to three independent groups of Sofjin strains of the Far Eastern subtype. Their genetic differences are not a result of microevolution associated with numerous passages. Retrospective analysis of the peculiarities of origin and distribution for each of these groups showed the authenticity of one of them. For the first time, we have determined a complete genome sequence of the authentic reference TBEV strain Sofjin. Two other groups of strains named Sofjin were probably the result of cross-contamination or laboratory error. The high probability of contamination requires the introduction of a new standard for virological laboratories, the key point of which is the obligatory genetic identification of all collection strains.
Starting Page	217
Ending Page	224
Page Count	8
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	44
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2011-11-18
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tick-borne encephalitis virus Retrospective analysis Authenticity Collection strain Laboratory error Medical Microbiology Plant Sciences Virology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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