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| Content Provider | Springer Nature Link |
|---|---|
| Author | Young, L. J. Deane, E. M. |
| Copyright Year | 2007 |
| Abstract | We describe the culture and stimulation of lymphocytes from the model marsupial, the tammar wallaby (Macropus eugenii). We also describe the capacity of tammar wallaby lymphocytes isolated from blood, spleen and lymph nodes to produce soluble immunomodulatory factors. Culture conditions were optimized for mitogen-driven stimulation using the plant lectin phytohaemagglutinin (PHA). Products secreted by stimulated cells were harvested and crudely fractionated before they were added back to freshly isolated lymphocytes. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay, both stimulatory and inhibitory bioactive factors were detected in serum-free supernatants harvested from mitogen-treated peripheral blood mononuclear cells. This paper describes the capacity of leukocytes of the tammar wallaby to respond to mitogenic stimulation and to produce soluble, low-molecular-weight bioactive molecules that possess cytokine-like activity. |
| Starting Page | 685 |
| Ending Page | 701 |
| Page Count | 17 |
| File Format | |
| ISSN | 01657380 |
| Journal | Veterinary Research Communications |
| Volume Number | 31 |
| Issue Number | 6 |
| e-ISSN | 15737446 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2007-01-23 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | bioactivity Macropus eugenii marsupial mitogen PBMC serum-free stimulation tammar wallaby Veterinary Medicine Zoology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary Medicine |
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