NDLI: Comparison of complement fixation test, competitive ELISA and LppQ ELISA with post-mortem findings in the diagnosis of contagious bovine pleuropneumonia (CBPP)

Content Provider	Springer Nature Link
Author	Muuka, Geofrey Hang’ombe, Bernard Mudenda Nalubamba, King Shimumbo Kabilika, Swithine Mwambazi, Lucas Muma, John Bwalya
Copyright Year	2011
Abstract	The complement fixation test (CFT), the c-ELISA and an indirect LppQ ELISA were compared to post-mortem (PM) inspection for the diagnosis of contagious bovine pleuropneumonia (CBPP). Sera from 797 cattle in the CBPP affected area of Kazungula, Zambia and 202 sera from Lusaka, Zambia, a CBPP-free area were used. The clinical history of CBPP was recorded and all the cattle from Kazungula were slaughtered and PM inspections conducted. The prevalence of CBPP in Kazungula was 67.5% (95%CI 67.2%, 70.8%), 52.6% (95%CI 49.2%, 56.2%), 59.0% (95%CI 55.5%, 62.4%) and 44.4% (95%CI 41.0%, 47.9%) using PM inspection, CFT, c-ELISA and LppQ ELISA, respectively. Three of the 202 negative control animals tested positive on the c-ELISA although they were from a known CBPP negative zone. In this study, the c-ELISA was more sensitive in detecting cattle with lesions in the chronic stage than any other test whilst the CFT detected more during the onset stage. No single serological test could detect all stages of CBPP infection, therefore the use of more than one test is advised.
Starting Page	1057
Ending Page	1062
Page Count	6
File Format	PDF
ISSN	00494747
Journal	Tropical Animal Health and Production
Volume Number	43
Issue Number	5
e-ISSN	15737438
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-02-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cattle CBPP c-ELISA CFT LppQ ELISA Post-mortem inspections Zambia Veterinary Medicine Zoology
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

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Comparison of complement fixation test, competitive ELISA and LppQ ELISA with post-mortem findings in the diagnosis of contagious bovine pleuropneumonia (CBPP).

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