NDLI: Alpha S1-casein polymorphisms in camel (Camelus dromedarius) and descriptions of biological active peptides and allergenic epitopes

Content Provider	Springer Nature Link
Author	Erhardt, Georg Shuiep, El Tahir Salih Lisson, Maria Weimann, Christina Wang, Zhaoxin El Zubeir, Ibtisam El Yas Mohamed Pauciullo, Alfredo
Copyright Year	2016
Abstract	Milk samples of 193 camels (Camelus dromedarius) from different regions of Sudan were screened for casein variability by isoelectric focusing. Kappa-casein and beta-casein were monomorphic, whereas three protein patterns named α$_{s1}$-casein A, C, and D were identified. The major allele A revealed frequencies of 0.79 (Lahaoi), 0.75 (Shanbali), 0.90 (Arabi Khali), and 0.88 (Arabi Gharbawi) in the different ecotypes. CSN1S1C shows a single G > T nucleotide substitution in the exon 5, leading to a non-synonymous amino acid exchange (p.Glu30 > Asp30) in comparison to CSN1S1A and D. At cDNA level, no further single nucleotide polymorphisms could be identified in CSN1S1* A, C, and D, whereas the variants CSN1S1A and CSN1S1C are characterized by missing of exon 18 compared to the already described CSN1S1*B, as consequence of DNA insertion of 11 bp at intron 17 which alter the pre-mRNA spliceosome machinery. A polymerase chain-restriction fragment length polymorphism method (PCR-RFLP) was established to type for G > T nucleotide substitution at genomic DNA level. The occurrence and differences of IgE-binding epitopes and bioactive peptides between α$_{s1}$-casein A, C, and D after digestion were analyzed in silico. The amino acid substitutions and deletion affected the arising peptide pattern and thus modifications between IgE-binding epitopes and bioactive peptides of the variants were found. The allergenic potential of these different peptides will be investigated by microarray immunoassay using sera from milk-sensitized individuals, as it was already demonstrated for bovine α$_{s1}$-casein variants.
Starting Page	879
Ending Page	887
Page Count	9
File Format	PDF
ISSN	00494747
Journal	Tropical Animal Health and Production
Volume Number	48
Issue Number	5
e-ISSN	15737438
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-02-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Milk proteins Genetic polymorphisms CSN1S1 IgE-binding epitopes Bioactive peptides Zoology Veterinary Medicine
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

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