NDLI: Evaluation of PCR test for detecting major pathogens of bubaline mastitis directly from mastitic milk samples of buffaloes

Content Provider	Springer Nature Link
Author	Anand Kumar, P.
Copyright Year	2009
Abstract	Present study is aimed to evaluate the PCR test for detecting the major pathogens of bubaline mastitis (BM) directly from the mastitic milk samples of the buffaloes. Cell lysates of the enriched mastitic milk samples were directly used as template DNA in PCR and the reactivity of genus and/or species specific primers against the selected pathogens of BM was tested in individual simplex PCR assays. Out of the 60 mastitic milk samples tested 30 were positive for E. coli, 21 were positive for S. aureus, 4 were positive for S. agalactiae, 9 were positive for S. dysgalactiae and 7 were positive for S. uberis. Certain samples were positive for more than one pathogen thus indicating the mixed infection of the udder. Although certain mastitic milk samples reacted with Staphylococcus genus specific primers, they didn’t react with S. aureus specific primers in PCR, which indicates the implication of other species of Staphylococcus in BM. The processing of mastitic milk samples was also simplified by eliminating the use of expensive reagents. The detection limits of PCR with the cell lysates are sensitive enough for PCR to be used as diagnostic tool for identifying the pathogens of BM.
Starting Page	1643
Ending Page	1651
Page Count	9
File Format	PDF
ISSN	00494747
Journal	Tropical Animal Health and Production
Volume Number	41
Issue Number	8
e-ISSN	15737438
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-05-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bubaline mastitis PCR Oligonucleotide primers Mastitic milk samples Cell lysates Detection limits Zoology Veterinary Medicine
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

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