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| Content Provider | Springer Nature Link |
|---|---|
| Author | Agrawal, Aditi Rajamani, Vijayalakshmi Reddy, Vanga Siva Mukherjee, Sunil Kumar Bhatnagar, Raj K. |
| Copyright Year | 2015 |
| Abstract | The success of Bt transgenics in controlling predation of crops has been tempered by sporadic emergence of resistance in targeted insect larvae. Such emerging threats have prompted the search for novel insecticidal molecules that are specific and could be expressed through plants. We have resorted to small RNA-based technology for an investigative search and focused our attention to an insect-specific miRNA that interferes with the insect molting process resulting in the death of the larvae. In this study, we report the designing of a vector that produces artificial microRNA (amiR), namely amiR-24, which targets the chitinase gene of Helicoverpa armigera. This vector was used as transgene in tobacco. Northern blot and real-time analysis revealed the high level expression of amiR-24 in transgenic tobacco plants. Larvae feeding on the transgenic plants ceased to molt further and eventually died. Our results demonstrate that transgenic tobacco plants can express amiR-24 insectice specific to H. armigera. |
| Starting Page | 791 |
| Ending Page | 801 |
| Page Count | 11 |
| File Format | |
| ISSN | 09628819 |
| Journal | Transgenic Research |
| Volume Number | 24 |
| Issue Number | 5 |
| e-ISSN | 15739368 |
| Language | English |
| Publisher | Springer International Publishing |
| Publisher Date | 2015-05-07 |
| Publisher Place | Cham |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Artificial miRNA Agrobacterium Transgenic tobacco Insecticidal Insect bioassay Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Transgenics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science |
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