NDLI: High level accumulation of α-glucan in maize kernels by expressing the gtfD gene from Streptococcus mutans

Content Provider	Springer Nature Link
Author	Zhang, Shirong Dong, Jian G. Wang, Tai Guo, Sherry Glassman, Kimberly Ranch, Jerry Nichols, Scott E.
Copyright Year	2006
Abstract	Glucosyltransferases (GTFs, EC.2.4.1.5) are bacterial enzymes that catalyze the polymerization of glucose residues from sucrose, leading to the production of high molecular weight glucan with α-1,3 /α-1,6 linkages. Such glucans, with many potential food and industrial applications, do not normally exist in higher plants. We fused a mutant form of the gtfD gene from Sreptococcus mutans with the maize (Zea mays L.) chloroplastic Brittle 1 transit peptide for amyloplast targeting. This construct, driven by the ubiquitin promoter, was introduced into maize by Agrobacterium-mediated transformation. We developed a novel HPLC-based method that enabled us differentially to distinguish transgene glucan from other endogenous polysaccharides in maize kernels. Using this method, we screened over 100 transgenic plants for the presence of GTF-produced glucan whose content varied between 0.8 and 14% of dry weight in the mature transgenic seeds. The mature transgenic plants were indistinguishable from wildtype plants in growth rate and morphology. Furthermore, starch granule size in the transgenic maize kernel was unaffected by the accumulation of the foreign polysaccharide. Mutation in Sh2, which encodes a subunit of ADP-glucose pyrophosphorylase, had no effect on glucan accumulation caused by gtfD expression. Our results indicated that high levels of novel carbohydrate polymer can be accumulated in crop plants through transgene technology.
Starting Page	467
Ending Page	478
Page Count	12
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	16
Issue Number	4
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-11-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Glucan Glucosyltransferase gtfD Transgenic maize Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

High level accumulation of α-glucan in maize kernels by expressing the gtfD gene from Streptococcus mutans

Transgenic maize plants expressing a fungal phytase gene

Expression of a Synthetic Porcine α-Lactalbumin Gene in the Kernels of Transgenic Maize

Lifelong stable human insulin expression in transgenic tilapia expressing a humanized tilapia insulin gene

Expression of a bacterial α-amylase gene in transgenic rice seeds

Production of transgenic dairy goat expressing human α-lactalbumin by somatic cell nuclear transfer

Transgenic maize endosperm containing a milk protein has improved amino acid balance

Expression of modified gene encoding functional human α-1-antitrypsin protein in transgenic tomato plants

Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease

High level accumulation of α-glucan in maize kernels by expressing the gtfD gene from Streptococcus mutans

Similar Documents

High level accumulation of α-glucan in maize kernels by expressing the gtfD gene from Streptococcus mutans

Transgenic maize plants expressing a fungal phytase gene

Expression of a Synthetic Porcine α-Lactalbumin Gene in the Kernels of Transgenic Maize

Lifelong stable human insulin expression in transgenic tilapia expressing a humanized tilapia insulin gene

Expression of a bacterial α-amylase gene in transgenic rice seeds

Production of transgenic dairy goat expressing human α-lactalbumin by somatic cell nuclear transfer

Transgenic maize endosperm containing a milk protein has improved amino acid balance

Expression of modified gene encoding functional human α-1-antitrypsin protein in transgenic tomato plants

Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease

High level accumulation of α-glucan in maize kernels by expressing the gtfD gene from Streptococcus mutans