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Enhancing gene targeting efficiency in higher plants: rice is on the move

Content Provider	Springer Nature Link
Author	Cotsaftis, Olivier Guiderdoni, Emmanuel
Copyright Year	2005
Abstract	Meeting the challenge of routine gene targeting (GT) in higher plants is of crucial interest to researchers and plant breeders who are currently in need of a powerful tool to specifically modify a given locus in a genome. Higher plants have long been considered the last lineage resistant to targeting technology. However, a recent report described an efficient method of T-DNA-mediated targeted disruption of a non-selectable locus in rice [Terada et al., Nat Biotechnol 20: 1030–1034 (2002)]. Though this study was an obvious breakthrough, further improvement of GT frequencies may derive from a better understanding of the natural mechanisms that control homologous recombination (HR) processes. In this review, we will focus on what is known about HR and the factors which may hamper the development of routine GT by HR in higher plants. We will also present the current strategies envisaged to overcome these limitations, such as expression of recombination proteins and refinements in the design of the transformation vector.
Starting Page	1
Ending Page	14
Page Count	14
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	14
Issue Number	1
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	allele replacement homologous recombination illegitimate recombination knockout positive and negative selection system recombination protein Human Genetics Plant Sciences Plant Genetics & Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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