NDLI: Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Please wait, while we are loading the content...

Transgenic accumulation of a defective cucumber mosaic virus (CMV) replicase derived double stranded RNA modulates plant defence against CMV strains O and Y in potato

Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Erratum to: Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Transformation of Althaea officinalis L. by Agrobacterium rhizogenes for the production of transgenic roots expressing the anti-HIV microbicide cyanovirin-N

Generation of c-Myc transgenic pigs for autosomal dominant polycystic kidney disease

Striatum-specific expression of Cre recombinase using the Gpr88 promoter in mice

Enhanced accumulation of atropine in Atropa belladonna transformed by Rac GTPase gene isolated from Scoparia dulcis

A new method for producing transgenic birds via direct in vivo transfection of primordial germ cells

Combining sperm plug genotyping and coat color chimerism predicts germline transmission

A novel chloroplast transformation vector compatible with the Gateway$^{®}$ recombination cloning technology

Transgenic Research : Volume 22, Issue 5, October 2013

Transgenic Research : Volume 22, Issue 4, August 2013

Transgenic Research : Volume 22, Issue 3, June 2013

Transgenic Research : Volume 22, Issue 2, April 2013

Transgenic Research : Volume 22, Issue 1, February 2013

Transgenic Research : Volume 21

Transgenic Research : Volume 20

Transgenic Research : Volume 19

Transgenic Research : Volume 18

Transgenic Research : Volume 17

Transgenic Research : Volume 16

Transgenic Research : Volume 15

Transgenic Research : Volume 14

Transgenic Research : Volume 13

Transgenic Research : Volume 12

Transgenic Research : Volume 11

Transgenic Research : Volume 10

Transgenic Research : Volume 9

Transgenic Research : Volume 8

Transgenic Research : Volume 7

Transgenic Research : Volume 6

Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Content Provider	Springer Nature Link
Author	Bachman, Pamela M. Bolognesi, Renata Moar, William J. Mueller, Geoffrey M. Paradise, Mark S. Ramaseshadri, Parthasarathy Tan, Jianguo Uffman, Joshua P. Warren, JoAnne Wiggins, B. Elizabeth Levine, Steven L.
Copyright Year	2013
Abstract	The sequence specificity of the endogenous RNA interference pathway allows targeted suppression of genes essential for insect survival and enables the development of durable and efficacious insecticidal products having a low likelihood to adversely impact non-target organisms. The spectrum of insecticidal activity of a 240 nucleotide (nt) dsRNA targeting the Snf7 ortholog in Western Corn Rootworm (WCR; Diabrotica virgifera virgifera) was characterized by selecting and testing insects based upon their phylogenetic relatedness to WCR. Insect species, representing 10 families and 4 Orders, were evaluated in subchronic or chronic diet bioassays that measured potential lethal and sublethal effects. When a specific species could not be tested in diet bioassays, the ortholog to the WCR Snf7 gene (DvSnf7) was cloned and corresponding dsRNAs were tested against WCR and Colorado potato beetle (Leptinotarsa decemlineata); model systems known to be sensitive to ingested dsRNA. Bioassay results demonstrate that the spectrum of activity for DvSnf7 is narrow and activity is only evident in a subset of beetles within the Galerucinae subfamily of Chrysomelidae (>90 % identity with WCR Snf7 240 nt). This approach allowed for evaluating the relationship between minimum shared nt sequence length and activity. A shared sequence length of ≥21 nt was required for efficacy against WCR (containing 221 potential 21-nt matches) and all active orthologs contained at least three 21 nt matches. These results also suggest that WCR resistance to DvSnf7 dsRNA due to single nucleotide polymorphisms in the target sequence of 240 nt is highly unlikely.
Starting Page	1207
Ending Page	1222
Page Count	16
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	22
Issue Number	6
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-06-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	RNAi Specificity Coleoptera Activity spectrum Non-target organism Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Erratum to: Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Erratum to: Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Characterization of the Spectrum of Activity of IPD079Ea: A Protein Derived From Ophioglossum pendulum (Ophioglossales: Ophioglossaceae) With Activity Against Western Corn Rootworm [Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)]

Characterizing the Mechanism of Action of Double-Stranded RNA Activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Western corn rootworm (Diabrotica virgifera virgifera) transcriptome assembly and genomic analysis of population structure

EFFICACY OF TRANSGENIC CORN EVENTS AGAINST CORN ROOTWORM LARVAE, 1999

LABORATORY EVALUATION OF ABAMECTIN AGAINST WESTERN CORN ROOTWORM ADULTS, 1999

GRANULAR AND LIQUID INSECTICIDE PERFORMANCE AGAINST CORN ROOTWORMS, 1998

Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Similar Documents

Erratum to: Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Erratum to: Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Characterization of the Spectrum of Activity of IPD079Ea: A Protein Derived From Ophioglossum pendulum (Ophioglossales: Ophioglossaceae) With Activity Against Western Corn Rootworm [Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)]

Characterizing the Mechanism of Action of Double-Stranded RNA Activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)

Western corn rootworm (Diabrotica virgifera virgifera) transcriptome assembly and genomic analysis of population structure

EFFICACY OF TRANSGENIC CORN EVENTS AGAINST CORN ROOTWORM LARVAE, 1999

LABORATORY EVALUATION OF ABAMECTIN AGAINST WESTERN CORN ROOTWORM ADULTS, 1999

GRANULAR AND LIQUID INSECTICIDE PERFORMANCE AGAINST CORN ROOTWORMS, 1998

Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)