NDLI: PORE: a modular binary vector series suited for both monocot and dicot plant transformation

Content Provider	Springer Nature Link
Author	Coutu, Catherine Brandle, James Brown, Dan Brown, Kirk Miki, Brian Simmonds, John Hegedus, Dwayne D.
Copyright Year	2007
Abstract	We present a series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotyledonous plants. The vector size has been minimized by eliminating all non-essential elements from the vector backbone and T-DNA regions while maintaining the ability to replicate independently. The smallest of the vector series is 6.3 kb and possesses an extensive multiple cloning site with 21 unique restriction endonuclease sites that are compatible with common cloning, protein expression, yeast two-hybrid and other binary vectors. The T-DNA region was engineered using a synthetic designer oligonucleotide resulting in an entirely modular system whereby any vector element can be independently exchanged. The high copy number ColE1 origin of replication has been included to enhance plasmid yield in Escherichia coli. FRT recombination sites flank the selectable marker cassette regions and allow for in planta excision by FLP recombinase. The pORE series consists of three basic types; an ‘open’ set for general plant transformation, a ‘reporter’ set for promoter analysis and an ‘expression’ set for constitutive expression of transgenes. The sets comprise various combinations of promoters (P $_{HPL}$, P $_{ENTCUP2}$ and P $_{TAPADH}$), selectable markers (nptII and pat) and reporter genes (gusA and smgfp).
Starting Page	771
Ending Page	781
Page Count	11
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	16
Issue Number	6
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-02-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Agrobacterium tumefaciens Binary vector Plant transformation T-DNA Promoter Selectable marker Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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