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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kung, Yi Jung Yu, Tsong Ann Huang, Chiung Huei Wang, Hui Chin Wang, Shin Lan Yeh, Shyi Dong |
| Copyright Year | 2009 |
| Abstract | Papaya production is seriously limited by Papaya ringspot virus (PRSV) worldwide and Papaya leaf-distortion mosaic virus (PLDMV) in Eastern Asia. An efficient transformation method for developing papaya lines with transgenic resistance to these viruses and commercially desirable traits, such as hermaphroditism, is crucial to shorten the breeding program for this fruit crop. In this investigation, an untranslatable chimeric construct pYP08 containing truncated PRSV coat protein (CP) and PLDMV CP genes coupled with the 3′ untranslational region of PLDMV, was generated. Root segments from different portions of adventitious roots of in vitro multiple shoots of hermaphroditic plants of papaya cultivars ‘Tainung No. 2’, ‘Sunrise’, and ‘Thailand’ were cultured on induction medium for regeneration into somatic embryos. The highest frequency of somatic embryogenesis was from the root-tip segments of adventitious roots developed 2–4 weeks after rooting in perlite medium. After proliferation, embryogenic tissues derived from somatic embryos were wounded in liquid-phase by carborundum and transformed by Agrobacterium carrying pYP08. Similarly, another construct pBG-PLDMVstop containing untranslatable CP gene of PLDMV was also transferred to ‘Sunrise’ and ‘Thailand’, the parental cultivars of ‘Tainung No. 2’. Among 107 transgenic lines regenerated from 349 root-tip segments, nine lines of Tainung No. 2 carrying YP08 were highly resistant to PRSV and PLDMV, and 9 lines (8 ‘Sunrise’ and 1 ‘Thailand’) carrying PLDMV CP highly resistant to PLDMV, by a mechanism of post-transcriptional gene silencing. The hermaphroditic characteristics of the transgenic lines were confirmed by PCR with sex-linked primers and phenotypes of flower and fruit. Our approach has generated transgenic resistance to both PRSV and PLDMV with commercially desirable characters and can significantly shorten the time-consuming breeding programs for the generation of elite cultivars of papaya hybrids. |
| Starting Page | 621 |
| Ending Page | 635 |
| Page Count | 15 |
| File Format | |
| ISSN | 09628819 |
| Journal | Transgenic Research |
| Volume Number | 19 |
| Issue Number | 4 |
| e-ISSN | 15739368 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2009-11-27 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Papaya transformation Hermaphrodite papaya Somatic embryos Adventitious roots Papaya ringspot virus Papaya leaf-distortion mosaic virus Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science |
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