NDLI: REVIEW: Spermatozoa, DNA binding and transgenic animals

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Transgenic Research : Volume 7, Issue 2, March 1998

Transgenic Research : Volume 7, Issue 6, January 1998

Transgenic Research : Volume 7, Issue 1, January 1998

Transgenic Research : Volume 6

REVIEW: Spermatozoa, DNA binding and transgenic animals

Content Provider	Springer Nature Link
Author	Gandolfi, Fulvio
Copyright Year	1998
Abstract	The idea that sperm cells could be used as an effective tool for introducing exogenous DNA into an oocyte at fertilization is generally regarded with scepticism. However, in recent years, several investigators have been working on different aspects of this intriguing research topic. In the present review, their results are summarised and discussed. Sections have been dedicated to the way DNA molecules bind to spermatozoa of different species, to the events regulating such binding, to the fate of the DNA within sperm cells, and to the attempts made to produce transgenic animals with this method. The data available on the interaction between DNA and spermatozoa begin to explain how this event takes place and how it is regulated. However, the stable integration of exogenous genes into the genome of adult animals mediated by sperm cells is a very rare event, although several reports describe forms of partial success. Available evidence suggests that changes to the DNA molecules, oc curring mostly within the oocyte, represents the limiting step in the production of transgenic animals using spermatozoa as vectors of exogenous genes. At present there are not enough data to understand what happens to sperm-associated DNA upon its entrance into the oocyte at fertilization. Therefore, it has not yet been resolved whether sperm-mediated gene transfer is a possible way to manipulate the genome or if evolution has imposed some unsurpassable barriers to its use
Starting Page	147
Ending Page	155
Page Count	9
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	7
Issue Number	3
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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REVIEW: Spermatozoa, DNA binding and transgenic animals

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REVIEW: Spermatozoa, DNA binding and transgenic animals