NDLI: Enhanced resistance to stripe rust disease in transgenic wheat expressing the rice chitinase gene RC24

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Enhanced resistance to stripe rust disease in transgenic wheat expressing the rice chitinase gene RC24

Content Provider	Springer Nature Link
Author	Huang, Xuan Wang, Jian Du, Zhen Zhang, Chen Li, Lan Xu, Ziqin
Copyright Year	2013
Abstract	Stripe rust is a devastating fungal disease of wheat worldwide which is primarily caused by Puccinia striiformis f. sp tritici. Transgenic wheat (Triticum aestivum L.) expressing rice class chitinase gene RC24 were developed by particle bombardment of immature embryos and tested for resistance to Puccinia striiformis f.sp tritici. under greenhouse and field conditions. Putative transformants were selected on kanamycin-containing media. Polymease chain reaction indicated that RC24 was transferred into 17 transformants obtained from bombardment of 1,684 immature embryos. Integration of RC24 was confirmed by Southern blot with a RC24-labeled probe and expression of RC24 was verified by RT-PCR. Nine transgenic T$_{1}$ lines exhibited enhanced resistance to stripe rust infection with lines XN8 and BF4 showing the highest level of resistance. Southern blot hybridization confirmed the stable inheritance of RC24 in transgenic T$_{1}$ plants. Resistance to stripe rust in transgenic T$_{2}$ and T$_{3}$ XN8 and BF4 plants was confirmed over two consecutive years in the field. Increased yield (27–36 %) was recorded for transgenic T$_{2}$ and T$_{3}$ XN8 and BF4 plants compared to controls. These results suggest that rice class I chitinase RC24 can be used to engineer stripe rust resistance in wheat.
Starting Page	939
Ending Page	947
Page Count	9
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	22
Issue Number	5
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-03-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chitinase Puccinia striformis f.sp tritici Stripe rust Transformation Wheat (Triticum aestivum L.) Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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