NDLI: Tissue Specific High Level Expression of a Full Length Human Endogenous Retrovirus Genome Transgene, HERV-R, Under Control of its Own Promoter in Rats

Content Provider	Springer Nature Link
Author	Tanaka, Satoshi Ikeda, Hitoshi Otsuka, riyuki Yamamoto, Yukiyo Sugaya, Toshiaki Yoshiki, Takashi
Copyright Year	2003
Abstract	Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under control of its own long terminal repeat (LTR) promoter. One line with tandem integration of multiple copies of the transgene expressed HERV-R mRNA in various organs with different expression levels and relatively higher in Harderian and submandibular salivary glands. In another line, the transgene was integrated as a single copy in a haploid and the expression was detected only in Harderian and submandibular salivary glands. In the placenta, one of the tissues with high levels of the HERV-R expression in humans, the transcription was evident starting the 12th day after gestation. A rabbit antiserum against synthetic peptides corresponding with the HERV-R env gene sequence led to detection of an 85 kDa product as a glycoprotein in the Harderian glands. While no pathological significance was observed in either line, the transgenic rat may prove to be a suitable model for analyzing the role of HERV-R function in vivo.
Starting Page	319
Ending Page	328
Page Count	10
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	12
Issue Number	3
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2003-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Kidney-Specific Activity of the Bovine Uromodulin Promoter

Expression of full-length bioactive antimicrobial human lactoferrin in potato plants

Conditional Transgene Expression in Endothelial Cells

Chromatin Heterogeneity Within Multicopy Transgene Arrays

Do transgene arrays form heterochromatin in vertebrates?

Promoter/leader deletion analysis and plant expression vectors with the figwort mosaic virus (FMV) full length transcript (FLt) promoter containing single or double enhancer domains

Tissue-specific expression of human salivary mucin gene, MUC7, in transgenic mice

Transgene Expression in the Vegetative Tissues of Apple Driven by the Vascular-specific rolC and CoYMV Promoters

Use of epitope tags for routine analysis of transgene expression

Tissue Specific High Level Expression of a Full Length Human Endogenous Retrovirus Genome Transgene, HERV-R, Under Control of its Own Promoter in Rats

Similar Documents

Kidney-Specific Activity of the Bovine Uromodulin Promoter

Expression of full-length bioactive antimicrobial human lactoferrin in potato plants

Conditional Transgene Expression in Endothelial Cells

Chromatin Heterogeneity Within Multicopy Transgene Arrays

Do transgene arrays form heterochromatin in vertebrates?

Promoter/leader deletion analysis and plant expression vectors with the figwort mosaic virus (FMV) full length transcript (FLt) promoter containing single or double enhancer domains

Tissue-specific expression of human salivary mucin gene, MUC7, in transgenic mice

Transgene Expression in the Vegetative Tissues of Apple Driven by the Vascular-specific rolC and CoYMV Promoters

Use of epitope tags for routine analysis of transgene expression

Tissue Specific High Level Expression of a Full Length Human Endogenous Retrovirus Genome Transgene, HERV-R, Under Control of its Own Promoter in Rats