NDLI: Genomic integration of adenoviral gene transfer vectors following transduction of fertilized mouse oocytes

Content Provider	Springer Nature Link
Author	Larochelle, Nancy Stucka, Rolf Rieger, rman Schermelleh, Lothar Schiedner, Gudrun Kochanek, Stefan Wolf, Eckhard Lochmüller, Hanns
Copyright Year	2010
Abstract	Adenoviral vectors (AdV) are popular tools to deliver foreign genes into a wide range of cells. They have also been used in clinical gene therapy trials. Studies on AdV-mediated gene transfer to mammalian oocytes and transmission through the germ line have been reported controversially. In the present study we investigated whether AdV sequences integrate into the mouse genome by microinjecting AdV into the perivitelline space of fertilized oocytes. We applied a newly developed PCR technique (HiLo-PCR) for identification of chromosomal junctions next to the integrated AdV. We demonstrate that mouse oocytes can be transduced by different recombinant adenoviral vectors (first generation and gutless). In one transgenic mouse line using the first generation adenoviral vector, the genome has integrated into a highly repetitive cluster located on the Y chromosome. While the transgene (GFP) was expressed in early embryos, no expression was detected in adult transgenic mice. The use of gutless AdV resulted in expression of the transgene, albeit the vector was not transmitted to progeny. These results indicate that under optimized conditions fertilized mouse oocytes are transduced by AdV and give rise to transgenic founder animals. Therefore, adequate precautions should be taken in gene therapy protocols of reproductive patients since transduction of oocytes or early embryos and subsequent chromosomal integration cannot be ruled out entirely.
Starting Page	123
Ending Page	135
Page Count	13
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	20
Issue Number	1
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-05-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adenovirus Transgenic mice Integration Oocytes Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A new method to efficiently produce transgenic embryos and mice from low-titer lentiviral vectors

Mechanism of random integration of foreign DNA in transgenic mice

Mice transgenic with SV40-late-promoter-driven Polyomavirus Middle T oncogene exclusively develop hemangiomas

Rapid generation of dendritic cell specific transgenic mice by lentiviral vectors

A survey to establish performance standards for the production of transgenic mice

Generating transgenic mice from bacterial artificial chromosomes: transgenesis efficiency, integration and expression outcomes

Skeletal defects in Osterix-Cre transgenic mice

Transgenic embryos and mice produced from low titre lentiviral vectors

Combining fibroblast isolation with lentiviral gene transfer to validate transgene expression in mice following pronucleus injection

Genomic integration of adenoviral gene transfer vectors following transduction of fertilized mouse oocytes

Similar Documents

A new method to efficiently produce transgenic embryos and mice from low-titer lentiviral vectors

Mechanism of random integration of foreign DNA in transgenic mice

Mice transgenic with SV40-late-promoter-driven Polyomavirus Middle T oncogene exclusively develop hemangiomas

Rapid generation of dendritic cell specific transgenic mice by lentiviral vectors

A survey to establish performance standards for the production of transgenic mice

Generating transgenic mice from bacterial artificial chromosomes: transgenesis efficiency, integration and expression outcomes

Skeletal defects in Osterix-Cre transgenic mice

Transgenic embryos and mice produced from low titre lentiviral vectors

Combining fibroblast isolation with lentiviral gene transfer to validate transgene expression in mice following pronucleus injection

Genomic integration of adenoviral gene transfer vectors following transduction of fertilized mouse oocytes