NDLI: RNA-interference in rice against Rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants

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RNA-interference in rice against Rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants

Content Provider	Springer Nature Link
Author	Tyagi, Himani Rajasubramaniam, Shanmugam Rajam, Manchikatla Venkat Dasgupta, Indranil
Copyright Year	2008
Abstract	Rice tungro is a viral disease seriously affecting rice production in South and Southeast Asia. Tungro is caused by the simultaneous infection in rice of Rice tungro bacilliform virus (RTBV), a double-stranded DNA virus and Rice tungro spherical virus (RTSV), a single-stranded RNA virus. To apply the concept of RNA-interference (RNAi) for the control of RTBV infection, transgenic rice plants expressing DNA encoding ORF IV of RTBV, both in sense as well as in anti-sense orientation, resulting in the formation of double-stranded (ds) RNA, were raised. RNA blot analysis of two representative lines indicated specific degradation of the transgene transcripts and the accumulation of small molecular weight RNA, a hallmark for RNA-interference. In the two transgenic lines expressing ds-RNA, different resistance responses were observed against RTBV. In one of the above lines (RTBV-O-Ds1), there was an initial rapid buildup of RTBV levels following inoculation, comparable to that of untransformed controls, followed by a sharp reduction, resulting in approximately 50-fold lower viral titers, whereas the untransformed controls maintained high levels of the virus till 40 days post-inoculation (dpi). In RTBV-O-Ds2, RTBV DNA levels gradually rose from an initial low to almost 60% levels of the control by 40 dpi. Line RTBV-O-Ds1 showed symptoms of tungro similar to the untransformed control lines, whereas line RTBV-O-Ds2 showed extremely mild symptoms.
Starting Page	897
Ending Page	904
Page Count	8
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	17
Issue Number	5
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-02-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Transgenic rice Rice tungro bacilliform virus RNAi Small RNA Green leafhopper Virus resistance Human Genetics Plant Sciences Animal Genetics and Genomics Plant Genetics & Genomics Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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RNA-interference in rice against Rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants

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RNA-interference in rice against Rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants

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RNA-interference in rice against Rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants