NDLI: Targeted modification of storage protein content resulting in improved amino acid composition of barley grain

Content Provider	Springer Nature Link
Author	Sikdar, Md. S. I. Bowra, S. Schmidt, D. Dionisio, G. Holm, P. B. Vincze, E.
Copyright Year	2015
Abstract	C-hordein in barley and ω-gliadins in wheat are members of the prolamins protein families. Prolamins are the major component of cereal storage proteins and composed of non-essential amino acids (AA) such as proline and glutamine therefore have low nutritional value. Using double stranded RNAi silencing technology directed towards C-hordein we obtained transgenic barley lines with up to 94.7 % reduction in the levels of C-hordein protein relative to the parental line. The composition of the prolamin fraction of the barley parental line cv. Golden Promise was resolved using SDS-PAGE electrophoresis, the protein band were excised and the proteins identified by quadrupole-time-of-flight mass spectrometry. Subsequent SDS-PAGE separation and analysis of the prolamin fraction of the transgenic lines revealed a reduction in the amounts of C-hordeins and increases in the content of other hordein family members. Analysis of the AA composition of the transgenic lines showed that the level of essential amino acids increased with a concomitant reduction in proline and glutamine. Both the barley C-hordein and wheat ω-gliadin genes proved successful for RNAi-gene mediated suppression of barley C-hordein level. All transgenic lines that exhibited a reduction for C-hordein showed off-target effects: the lines exhibited increased level of B/γ-hordein while D-hordein level was reduced. Furthermore, the multicopy insertions correlated negatively with silencing.
Starting Page	19
Ending Page	31
Page Count	13
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	25
Issue Number	1
e-ISSN	15739368
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-10-27
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antisense transgenic plants Cereal storage proteins Grain protein composition Mass spectrometry Alteration multigene families Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Transgenics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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