NDLI: Expression of the nos operon proteins from Pseudomonas stutzeri in transgenic plants to assemble nitrous oxide reductase

Content Provider	Springer Nature Link
Author	Wan, Shen Mottiar, Yaseen Johnson, Amanda M. Goto, Kagami Altosaar, Illimar
Copyright Year	2011
Abstract	Nitrous oxide (N$_{2}$O) is a stable greenhouse gas that plays a significant role in the destruction of the ozone layer. Soils are a significant source of atmospheric N$_{2}$O. It is important to explore some innovative and effective biology-based strategies for N$_{2}$O mitigation. The enzyme nitrous oxide reductase (N$_{2}$OR), naturally found in soil bacteria, is responsible for catalysing the final step of the denitrification pathway, conversion of N$_{2}$O to dintrogen gas (N$_{2}$). To transfer this catalytic pathway from soil into plants and amplify the abundance of this essential mechanism (to reduce global warming), a mega-cassette of five coding sequences was assembled to produce transgenic plants heterologously expressing the bacterial nos operon in plant leaves. Both the single-gene transformants (nosZ) and the multi-gene transformants (nosFLZDY) produced active recombinant N$_{2}$OR. Enzymatic activity was detected using the methyl viologen-linked enzyme assay, showing that extracts from both types of transgenic plants exhibited N$_{2}$O-reducing activity. Remarkably, the single-gene strategy produced higher reductase capability than the whole-operon approach. The data indicate that bacterial N$_{2}$OR expressed in plants could convert N$_{2}$O into inert N$_{2}$ without involvement of other Nos proteins. Silencing by homologous signal sequences, or cryptic intracellular targeting are possible explanations for the low activities obtained. Expressing N$_{2}$OR from Pseudomonas stutzeri in single-gene transgenic plants indicated that such ag-biotech solutions to climate change have the potential to be easily incorporated into existing genetically modified organism seed germplasm.
Starting Page	593
Ending Page	603
Page Count	11
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	21
Issue Number	3
e-ISSN	15739368
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-09-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nitrous oxide Greenhouse gas Global warming Fertilizer Nitrous oxide reductase ABC transporter Plant Sciences Plant Genetics & Genomics Molecular Medicine Animal Genetics and Genomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Phytoremediation of Nitrous Oxide: Expression of Nitrous Oxide Reductase from Pseudomonas Stutzeri in Transgenic Plants and Activity thereof

Operon Structure and Regulation of the nos Gene Region of Pseudomonas stutzeri, Encoding an ABC-Type ATPase for Maturation of Nitrous Oxide Reductase

Production of dextran in transgenic potato plants

Greenhouse Gas Fluxes from Sugarcane and Pigeon Pea Cultivated Soils

Monitoring the Spread of Recombinant DNA from Field Plots with Transgenic Sugar Beet Plants by PCR and Natural Transformation of Pseudomonas Stutzeri

Expression of Functional Interleukin-12 from Mouse in Transgenic Tomato Plants

Expression of a functional human adenosine deaminase in transgenic tobacco plants

Lignin biosynthesis in transgenic Norway spruce plants harboring an antisense construct for cinnamoyl CoA reductase (CCR)

Transgenic maize plants expressing a fungal phytase gene

Expression of the nos operon proteins from Pseudomonas stutzeri in transgenic plants to assemble nitrous oxide reductase

Similar Documents

Phytoremediation of Nitrous Oxide: Expression of Nitrous Oxide Reductase from Pseudomonas Stutzeri in Transgenic Plants and Activity thereof

Operon Structure and Regulation of the nos Gene Region of Pseudomonas stutzeri, Encoding an ABC-Type ATPase for Maturation of Nitrous Oxide Reductase

Production of dextran in transgenic potato plants

Greenhouse Gas Fluxes from Sugarcane and Pigeon Pea Cultivated Soils

Monitoring the Spread of Recombinant DNA from Field Plots with Transgenic Sugar Beet Plants by PCR and Natural Transformation of Pseudomonas Stutzeri

Expression of Functional Interleukin-12 from Mouse in Transgenic Tomato Plants

Expression of a functional human adenosine deaminase in transgenic tobacco plants

Lignin biosynthesis in transgenic Norway spruce plants harboring an antisense construct for cinnamoyl CoA reductase (CCR)

Transgenic maize plants expressing a fungal phytase gene

Expression of the nos operon proteins from Pseudomonas stutzeri in transgenic plants to assemble nitrous oxide reductase