NDLI: Recombinant Human Protein C Expression in the Milk of Transgenic Pigs and the Effect on Endogenous Milk Immunoglobulin and Transferrin Levels

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Recombinant Human Protein C Expression in the Milk of Transgenic Pigs and the Effect on Endogenous Milk Immunoglobulin and Transferrin Levels

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Acknowledgement ( Transgenic Research , Volume 10 , Issue 1 )

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Transgenic Research : Volume 6

Recombinant Human Protein C Expression in the Milk of Transgenic Pigs and the Effect on Endogenous Milk Immunoglobulin and Transferrin Levels

Content Provider	Springer Nature Link
Author	Van Cott, Kevin E. Lubon, Henryk Gwazdauskas, F.C. Knight, James Drohan, William N. Velander, William H.
Copyright Year	2001
Abstract	Colostrum and milk are natural vehicles for acquiring passive immunity and are valuable tools for decreasing neonatant mortality from diarrheal disease. The effects of recombinant human protein C (rhPC) expression levels on endogenous immunoglobulin and transferrin content of the milk of different lineages of transgenic pigs were studied. The levels of rhPC in the milk ranged from 40 to 1200 μg/ml. Transgenic pigs with rhPC expression levels less than 500 μg/ml had no significant differences in milk protein composition with respect to nontransgenic pigs. A line of transgenic pigs having rhPC expression levels of 960–1200 μg/ml had two- to three-fold higher IgG, IgM, and secretory IgA concentrations compared to other transgenic and nontransgenic pig groups (P < 0.05), and four- to five-fold higher transferrin levels than nontransgenic pigs (P < 0.05). Changes in milk protein composition were not associated with mastitis or other pathologic disruption of epithelial cell junctions as indicated by normal casein and albumin levels in milk. Since IgG, IgM, secretory IgA, and transferrin are transported into the milk by transcytosis, higher levels of these proteins indicate that transcyctosis in the mammary epithelial cell was likely upregulated in pigs having high rhPC expression levels. This study is the first that shows a statistically significant example that mammary tissue specific expression of a heterologous protein can enhance endogenous phenotypic characteristics of milk.
Starting Page	43
Ending Page	51
Page Count	9
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	10
Issue Number	1
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2001-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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