NDLI: Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post‐transcriptional gene silencing

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Transgenic Research : Volume 8, Issue 2, April 1999

Transgenic Research : Volume 8, Issue 1, February 1999

Transgenic Research : Volume 7

Transgenic Research : Volume 6

Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post‐transcriptional gene silencing

Content Provider	Springer Nature Link
Author	Jan, Fuh‐Jyh Pang, Sheng‐Zhi Fagoaga, Carmen Gonsalves, Dennise
Copyright Year	1999
Abstract	The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV‐ESC8) was cloned and sequenced. Comparisons of the 867‐nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7–89.3% nucleotide identity and 92.4–95.5% amino acid identity. The CP gene was cloned into a plant expression vector and transformed into Nicotiana benthamiana plants via Agrobacterium tumefaciens‐mediated leaf disk transformation. Progeny from R$_{0}$ lines was screened for resistance to TuMV‐ESC8. Five of 29 tested lines showed TuMV protection in more than 50% of their progeny. Interestingly, some of the resistant plants transformed with the CP gene of TuMV displayed mild mosaicism in the new growing leaves at the later stages of evaluation; but these mosaic symptoms disappeared when the leaves were fully expanded. Collective data from steady‐state RNA analysis and nuclear run‐on assay of a line showed that the resistance was RNA‐mediated through the post‐transcriptional gene silencing mechanism.
Starting Page	203
Ending Page	213
Page Count	11
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	8
Issue Number	3
e-ISSN	15739368
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1999-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Plant Sciences Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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