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| Content Provider | Springer Nature Link |
|---|---|
| Author | Schneider, Anja Stelljes, Christian Adams, Caroline Kirchner, Stefan Burkhard, Gabi Jarzombski, Sabine Broer, Inge Horn, Patricia Elsayed, Ashraf Hagl, Peter Leister, Dario Koop, Hans Ulrich |
| Copyright Year | 2014 |
| Abstract | Plastid-encoded genes are maternally inherited in most plant species. Transgenes located on the plastid genome are thus within a natural confinement system, preventing their distribution via pollen. However, a low-frequency leakage of plastids via pollen seems to be universal in plants. Here we report that a very low-level paternal inheritance in Arabidopsis thaliana occurs under field conditions. As pollen donor an Arabidopsis accession (Ler-Ely) was used, which carried a plastid-localized atrazine resistance due to a point mutation in the psbA gene. The frequency of pollen transmission into F1 plants, based on their ability to express the atrazine resistance was 1.9 × 10$^{−5}$. We extended our analysis to another cruciferous species, the world-wide cultivated crop Brassica napus. First, we isolated a fertile and stable plastid transformant (T36) in a commercial cultivar of B. napus (cv Drakkar). In T36 the aadA and the bar genes were integrated in the inverted repeat region of the B. napus plastid DNA following particle bombardment of hypocotyl segments. Southern blot analysis confirmed transgene integration and homoplasmy of plastid DNA. Line T36 expressed Basta resistance from the inserted bar gene and this trait was used to estimate the frequency of pollen transmission into F1 plants. A frequency of <2.6 × 10$^{−5}$ was determined in the greenhouse. Taken together, our data show a very low rate of paternal plastid transmission in Brassicacea. Moreover, the establishment of plastid transformation in B. napus facilitates a safe use of this important crop plant for plant biotechnology. |
| Starting Page | 267 |
| Ending Page | 277 |
| Page Count | 11 |
| File Format | |
| ISSN | 09628819 |
| Journal | Transgenic Research |
| Volume Number | 24 |
| Issue Number | 2 |
| e-ISSN | 15739368 |
| Language | English |
| Publisher | Springer International Publishing |
| Publisher Date | 2014-10-25 |
| Publisher Place | Cham |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Arabidopsis thaliana Brassica napus Glufosinate resistance Paternal inheritance Plastid transformation Plastid inheritance Transgene containment Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Transgenics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science |
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