NDLI: The C-terminus of DSX$^{F5}$ protein acts as a novel regulatory domain in Bombyx mori

Content Provider	Springer Nature Link
Author	Duan, Jianping Meng, Xianxin Ma, Sanyuan Wang, Feng Guo, Huozhen Zhang, Liying Zhao, Ping Kan, Yunchao Yao, Lunguang Xia, Qingyou
Copyright Year	2016
Abstract	The doublesex gene regulates the somatic sexual development of Bombyx mori by alternatively splicing into sex-specific splice forms. In our previous study, the splice form Bmdsx $^{ F7 }$, which encodes the BmDSX$^{F5}$ protein, was found to be expressed in a female-specific manner and to contain a novel C-terminus. In this study, we aimed to investigate the role of this C-terminus. Two transgenic lines, L1 and L2, were constructed to ectopically express Bmdsx $^{ F7 }$ in males. Phenotype and W chromosome-specific polymerase chain reaction (PCR) analysis showed that developmental abnormalities and sex reversal did not occur. Moreover, the sex ratio was also normal. Quantitative PCR revealed that the expression levels of SP1 and Vg were upregulated in the fat body of transgenic males. Additionally, the expression level of PBP was downregulated in the antenna of transgenic males. The results suggested that the C-terminus of BmDSX$^{F5}$ functioned as a regulatory domain during regulation of downstream target gene expression and that BmDSX$^{F5}$ participated in the sexual development of somatic cells together with other DSX proteins in B. mori.
Starting Page	491
Ending Page	497
Page Count	7
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	25
Issue Number	4
e-ISSN	15739368
Language	English
Publisher	Springer International Publishing
Publisher Date	2016-03-15
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bombyx mori Doublesex gene C-terminus Transgene Target genes Animal Genetics and Genomics Plant Genetics & Genomics Transgenics Biomedical Engineering/Biotechnology Genetic Engineering Molecular Medicine
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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