NDLI: Optimized culture conditions for the production of furanocoumarins by micropropagated shoots of Ruta graveolens

Content Provider	Springer Nature Link
Author	Massot, B. Milesi, S. Gontier, E. Bourgaud, F. Guckert, A.
Copyright Year	2000
Abstract	Ruta graveolens in vitro cultures are a potential source of secondary metabolites (furanocoumarins) of significant medical interest. Experiments led in our laboratory showed that micropropagated shoots were richer in furanocoumarins than any other plant material. In order to optimize the molecule production by such cultivation systems, several factors related to the culture medium were studied. Effects of medium composition on biomass growth and furanocoumarin content were analysed and optimal conditions were determined for phosphate (300 mg l$^{−1}$ of NaH$_{2}$PO$_{4}$), nitrate (2527 mg l$^{−1}$ of KNO$_{3}$), carbon source (10 g l$^{−1}$ of sucrose) and phytohormones (2,4-dichlorophenoxyacetic acid (2,4-D) 50 μM and benzylaminopurine (BAP) 50 μM). Ruta shoot growth and furanocoumarin production were compared for optimized and standard culture conditions. Specific medium gave better results in terms of growth (t$_{D}$ equal to 6.9 days against 8.6 for standard conditions) but no significant differences appeared concerning metabolite concentrations. However, the present study opens the way to scale-up studies with bioreactor cultivation systems.
Starting Page	11
Ending Page	19
Page Count	9
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	62
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2000-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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