NDLI: Callus induction and shoot organogenesis from anther cultures of Curcuma attenuata Wall

Content Provider	Springer Nature Link
Author	Kou, Yaping Ma, Guohua Teixeira da Silva, Jaime A. Liu, Nian
Copyright Year	2012
Abstract	Curcuma attenuata is a highly valued ornamental. This study provides the first report on C. attenuata shoot organogenesis and plant regeneration. Immature anthers derived from 5 to 7 cm long inflorescences were isolated and cultured on different variations of Murashige and Skoog (MS) media to induce callus and then shoot organogenesis. When the 2-mm long anthers in which microspores were at the uninucleate developmental stage were cultured in the dark on MS medium containing 13.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.3 μM kinetin (KT) for 15 days and then transferred to 40 μmol m$^{−2}$ s$^{−1}$ fluorescent light for 30 days, the percentage callus induction reached 33.3 %. After callus was transferred to various differentiation media and cultured in the light, 33.1 % of all callus cultures could differentiate into adventitious shoots on MS medium supplemented with 22.0 μM 6-benzyladenine (BA), 0.53 μM α-naphthaleneacetic acid (NAA) and 1.4 μM thidiazuron (TDZ) after culturing for 60 days. Over 95 % of plantlets survived after transplanting plantlets into trays with a mixture of sand and perlite (2: 1) for 20 days. Chromosome number, determined from the root tips of young plantlets, indicated that all plantlets were diploid (2n = 84).
Starting Page	1
Ending Page	7
Page Count	7
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	112
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-07-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Callus differentiation Plant regeneration Shoot propagation Root formation Transplanting Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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